Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000915_6j71 ACATATTTAAACTGTGAGTAGCGACTCCTCGG(+ACTCAC)GTACCTTTCAGTACTGTGTGTACAAACCACT NGG 10 8 17 8 24 0 100 5 10
    240605000913_b6i6 CTGACAGACAGCCAAAATGGGGCAGGTTCCAG(+ACTCAC)GATCCTGATTGGTCGAGTGCTGGAGGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605000912_ogr5 GGCTCTGCCTCCTCCAGCACTCGACCAATCAG(+ACTCAC)GATCCTGGAACCTGCCCCATTTTGGCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605000911_jz6f AGCGTTTGCCAAGATGGAGCTTTATACAGATG(+ACTCAC)GTACCTCCTAAGTCCCCTCTCTGGCCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605000909_j72q ATGCAATGCTGGCCAGAGAGGGGACTTAGGAG(+ACTCAC)GTACCATCTGTATAAAGCTCCATCTTGGCAA NGG 10 8 17 8 24 0 100 5 10
    240605000907_64o0 GCATATATGTATGTAAGCAGACCACGTGGGTG(+ACTCAC)GTACCTGTAGGGTATCAGAAGAGGGCATCAG NGG 10 8 17 8 24 0 100 5 10
    240605000906_jbdt GGGATCTGATGCCCTCTTCTGATACCCTACAG(+ACTCAC)GTACCACCCACGTGGTCTGCTTACATACATA NGG 10 8 17 8 24 0 100 5 10
    240605000905_vjbz ACCCCCAGGAGAGAGTGGTGGAGAGAGTGGAG(+ACTCAC)GATCCCGCAGGTCTTTGCCAATTAAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240605000903_5sri TACCCCAAAGTTTAATTGGCAAAGACCTGCGG(+ACTCAC)GATCCTCCACTCTCTCCACCACTCTCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605000900_egjm AGAAAACAGCCTGGAAGGGTAAGTACCCCCGG(+ACTCAC)GTACCATACACTTCTGAAATCCCGAGCCTTT NGG 10 8 17 8 24 0 100 5 10