Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000424_n2ck AGCCAGAGTCAAACTGATCCTTTATACAGGGG(+ACTCAC)GTACCATGACAGTACAACAGTGTCGTTAACC NGG 10 8 17 8 24 0 100 5 10
    240605000423_wz7s GCAGGGAGAAATGTAGGGATGTCTTTGGACAG(+ACTCAC)GATCCTGTATACCTGTGGGAGCAGTGGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605000422_eile CTTTTTTCCCCACTGCTCCCACAGGTATACAG(+ACTCAC)GATCCTGTCCAAAGACATCCCTACATTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605000420_w1qg CAGTGAGCCCCTCTGTCTCCCACCAGTCTTGG(+ACTCAC)GTACCTCTCCCAGAGCTCCCCCAGGACTGAG NGG 10 8 17 8 24 0 100 5 10
    240605000419_5job CATGACTCAGTCCTGGGGGAGCTCTGGGAGAG(+ACTCAC)GTACCCAAGACTGGTGGGAGACAGAGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605000416_9reh TTTTCTGTACTGAAGAAGGGAGGGTGGGGAGG(+ACTCAC)GTACCCCTCTTTCAATGCCTGGTCTCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240605000414_n6lg TGGACATAAAGAGACCAGGCATTGAAAGAGGG(+ACTCAC)GTACCCTCCCCACCCTCCCTTCTTCAGTACA NGG 10 8 17 8 24 0 100 5 10
    240605000413_f1y0 CATAGAAACATGAAGCTCCCACGCAAGGCGTG(+ACTCAC)GATCCAAACAAAGCACAGACAACACTGAACG NGG 10 8 17 8 24 0 100 5 10
    240605000412_6ynm CTCCCCGTTCAGTGTTGTCTGTGCTTTGTTTG(+ACTCAC)GATCCACGCCTTGCGTGGGAGCTTCATGTTT NGG 10 8 17 8 24 0 100 5 10
    240605000410_p1ui AGGACTGGCACAAGTTAATGGGGAAGAGGAAG(+ACTCAC)GTACCGCAGAACGGAGCTAATCCGTCACTGT NGG 10 8 17 8 24 0 100 5 10