Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000350_0zpx GCCTTCTGCTCATACATCCACAGGGAAGGGCG(+ACTCAC)GATCCCTTTCTCATCACTGGGCTACCAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605000347_e2ze CAAAGCTGCAGGCTGGCATGGTCCCCACCAGG(+ACTCAC)GATCCTCTGGCCCATCCACGCTGTGCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240605000345_w35a GGCCGACCCTGCACAGCGTGGATGGGCCAGAG(+ACTCAC)GATCCCTGGTGGGGACCATGCCAGCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605000344_4acm GGTGTCCCATGCTCACTTCAGCACTGTTCAGG(+ACTCAC)GTACCTGACGGGGCAGTCCCACCCTCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240605000342_mm2h TGTTGGAACAGAGGGTGGGACTGCCCCGTCAG(+ACTCAC)GTACCCTGAACAGTGCTGAAGTGAGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240605000341_uvg4 CCTGCCATTGTTGGTTTGATGAACACGATTAG(+ACTCAC)GTACCCAGTGTGATCCCACCCTCACCCCACC NGG 10 8 17 8 24 0 100 5 10
    240605000339_y37s GGTGAGGTGGGGTGAGGGTGGGATCACACTGG(+ACTCAC)GTACCTAATCGTGTTCATCAAACCAACAATG NGG 10 8 17 8 24 0 100 5 10
    240605000337_skju TGAGTCTGTCCTCCTCCAAGGTCAGCCCTCGG(+ACTCAC)GATCCCAAAATAGTCCCTTCTGAAGGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240605000335_o4ct GTCAGCACCCCCTTCAGAAGGGACTATTTTGG(+ACTCAC)GATCCCGAGGGCTGACCTTGGAGGAGGACAG NGG 10 8 17 8 24 0 100 5 10
    240605000333_oxj5 CCCTGAGGCACGAGGGGACTGTGTTCTCAGGG(+ACTCAC)GATCCACCAGGATAGTGCCTGACTACAGATC NGG 10 8 17 8 24 0 100 5 10