Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000711_w101 TTTAACCTGCAGCAGAAAGATGTAAGCTGCAG(+ACTCAC)GTACCTCTAACTACAGGAAATGGCAGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605000709_isqb AACAATGTCCCTGACTCTGTTGCCTGCCTGAG(+ACTCAC)GATCCCATTCCCCTAACTGGACTGCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240605000707_jhmf GACCAGACAAGGCAGTCCAGTTAGGGGAATGG(+ACTCAC)GATCCTCAGGCAGGCAACAGAGTCAGGGACA NGG 10 8 17 8 24 0 100 5 10
    240605000705_0iy2 TGATAAAGATTCCTGTAAACTGGGATCACTGG(+ACTCAC)GATCCAGTGTGTGATCTAATCTTTACACAGG NGG 10 8 17 8 24 0 100 5 10
    240605000704_x2st GCACACCTGTGTAAAGATTAGATCACACACTG(+ACTCAC)GATCCCAGTGATCCCAGTTTACAGGAATCTT NGG 10 8 17 8 24 0 100 5 10
    240605000703_1tev GCAATGGTTGATCTTGGCTGTTGACCTCACTG(+ACTCAC)GATCCAGAACTAATAAAAATAAGACAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605000702_wa1g GGCAGCAGCTTGTCTTATTTTTATTAGTTCTG(+ACTCAC)GATCCAGTGAGGTCAACAGCCAAGATCAACC NGG 10 8 17 8 24 0 100 5 10
    240605000700_9gj2 TTGAGTTTCTCAAAATATAATATTTATTGTTG(+ACTCAC)GATCCATTATCTTAAAAGAATCATCCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605000659_jnjp AGTCTTCCCAGGATGATTCTTTTAAGATAATG(+ACTCAC)GATCCAACAATAAATATTATATTTTGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605000658_vfkg ACCTGTCACACACCTCCACCCCAAGGTTAAGG(+ACTCAC)GATCCAGGATGAAGACTCTAAGAGCCAGAAG NGG 10 8 17 8 24 0 100 5 10