Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000230_6n9j GGGAACAGCCCTAGTGAGCCAGAGTTAGTCAG(+ACTCAC)GTACCGCTTAGATGGAACCCGGTCTCATCAG NGG 10 8 17 8 24 0 100 5 10
    240605000228_g7y0 ACTAACTTGAGGTGAGGCAGCAAAAGGGCCTG(+ACTCAC)GTACCACCAAATGGTGGCTTCCACCCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240605000226_rar4 TGACCCCACGGGGTGGAAGCCACCATTTGGTG(+ACTCAC)GTACCAGGCCCTTTTGCTGCCTCACCTCAAG NGG 10 8 17 8 24 0 100 5 10
    240605000225_drcv AGACCAGCCTTCTCAGGTTCTCTGGCTCGTGG(+ACTCAC)GATCCTTCCAGGGAGCTACCTACAGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605000223_4doa TAACCCAAGCTCTGTAGGTAGCTCCCTGGAAG(+ACTCAC)GATCCCACGAGCCAGAGAACCTGAGAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605000221_6535 CCAAGGAATGGAAGGCCCAGGTCTTTCTCCAG(+ACTCAC)GTACCGTACAGCTGGCCCCTTTGCTTACTGC NGG 10 8 17 8 24 0 100 5 10
    240605000220_wp8o GCAGAGCAGTAAGCAAAGGGGCCAGCTGTACG(+ACTCAC)GTACCTGGAGAAAGACCTGGGCCTTCCATTC NGG 10 8 17 8 24 0 100 5 10
    240605000219_q6o9 TCAATGGTGCTTTAAGGACGTGTGCAGGATAG(+ACTCAC)GTACCTTAGAGGAGCAGCCTAGTTTCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605000218_chzp CATCAGCAGAGAAACTAGGCTGCTCCTCTAAG(+ACTCAC)GTACCTATCCTGCACACGTCCTTAAAGCACC NGG 10 8 17 8 24 0 100 5 10
    240605000216_ycjh GTCCTGTTAGATGGAATCCTGTCATCTCATTG(+ACTCAC)GTACCCAGGGAGCCCAACTTCTTAGTAGCTC NGG 10 8 17 8 24 0 100 5 10