Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000317_y2wg AAGTCTCTCTACTGTGCTCAGAGCAGTCCTTG(+ACTCAC)GTACCTGAGTGGTGCTAACAGCCCAGAACGG NGG 10 8 17 8 24 0 100 5 10
    240605000316_55j1 AGCCAACAGGAAAAACTACTGAAAATGTCATG(+ACTCAC)GATCCCATGTCAGTCTGGCACTTGATTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605000314_r09n GGCACAAAGAATCAAGTGCCAGACTGACATGG(+ACTCAC)GATCCATGACATTTTCAGTAGTTTTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605000312_i1h2 CACTAACCACAACACAGTACAAGTGTCTCTGG(+ACTCAC)GTACCCCGAACATCTCCCCGAAGAGTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240605000310_psbf GAGGACTAGAACTCTTCGGGGAGATGTTCGGG(+ACTCAC)GTACCCAGAGACACTTGTACTGTGTTGTGGT NGG 10 8 17 8 24 0 100 5 10
    240605000309_59qq ATATTCATGTTTATTCGCTCTCTACCAAGTAG(+ACTCAC)GATCCATTTTTGATAAAGCAGTCTTTGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605000308_79gr GAAGAAACCCAAAGACTGCTTTATCAAAAATG(+ACTCAC)GATCCTACTTGGTAGAGAGCGAATAAACATG NGG 10 8 17 8 24 0 100 5 10
    240605000306_39co GTGTGGTGCAACCTAGAGCCCATCAGAGGTGG(+ACTCAC)GTACCTATGTATACAAGTCCCACCTGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605000304_jxut CTGACAAAGCCAGGTGGGACTTGTATACATAG(+ACTCAC)GTACCCACCTCTGATGGGCTCTAGGTTGCAC NGG 10 8 17 8 24 0 100 5 10
    240605000302_y9qa ACTGGAAGCCCAAGCCAAACGCCCAAGCCTCG(+ACTCAC)GTACCTATTAGGACTTACACTGACCTCGCAC NGG 10 8 17 8 24 0 100 5 10