Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000301_yqka TCCGTGTGCGAGGTCAGTGTAAGTCCTAATAG(+ACTCAC)GTACCGAGGCTTGGGCGTTTGGCTTGGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605000300_j8lw TTTAAAAATATATATATAAACTAAATCACACG(+ACTCAC)GTACCAAAGCTGTAATGGAAAAAGGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605000259_gbod CTTAACTCTTTCCTTTTTCCATTACAGCTTTG(+ACTCAC)GTACCGTGTGATTTAGTTTATATATATATTT NGG 10 8 17 8 24 0 100 5 10
    240605000257_9i1i CAGCACATCCTAGGCAGGGCCGAACCAGCATG(+ACTCAC)GTACCAAGTGCAAAGATCCGACTTACCTACA NGG 10 8 17 8 24 0 100 5 10
    240605000256_fveq CGATCTGTAGGTAAGTCGGATCTTTGCACTTG(+ACTCAC)GTACCATGCTGGTTCGGCCCTGCCTAGGATG NGG 10 8 17 8 24 0 100 5 10
    240605000254_2un8 CCTCGTGTCCACCGCAGGCTTTTGGTACAAGG(+ACTCAC)GTACCTCATTTGCGTTTTTTAAAACCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605000253_i30x AAGGCAGACTGGTTTTAAAAAACGCAAATGAG(+ACTCAC)GTACCCTTGTACCAAAAGCCTGCGGTGGACA NGG 10 8 17 8 24 0 100 5 10
    240605000252_tbaz GAACACAAGGAAACTCAGTCATGCTCAGAAAG(+ACTCAC)GATCCAGCTAAGTGAGCAGGTGGACTCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605000250_e4ie TGAGCCTTGGAGTCCACCTGCTCACTTAGCTG(+ACTCAC)GATCCTTTCTGAGCATGACTGAGTTTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240605000249_b1x8 GCTGTTTTACAGCAGTGTGCCAAGAGATGTGG(+ACTCAC)GATCCACAGAGGGATTTTCTAACCTGGAGCA NGG 10 8 17 8 24 0 100 5 10