Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000214_h6vj CAAGAGAGCTACTAAGAAGTTGGGCTCCCTGG(+ACTCAC)GTACCAATGAGATGACAGGATTCCATCTAAC NGG 10 8 17 8 24 0 100 5 10
    240605000212_kq1z TGGGTAACTACGGAGGCCAAAAGAAGGCATTG(+ACTCAC)GATCCCCTGGAGCTGTAGTTACAGGTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605000210_hplh GCTCACAAGCACCTGTAACTACAGCTCCAGGG(+ACTCAC)GATCCAATGCCTTCTTTTGGCCTCCGTAGTT NGG 10 8 17 8 24 0 100 5 10
    240605000208_ilqi GGCAGGTGAGTAGCTCTGAGCTCCTGCCTCAG(+ACTCAC)GTACCTTAACATGGACAGCAATGTGGAAACT NGG 10 8 17 8 24 0 100 5 10
    240605000206_7xho ATTCCAGTTTCCACATTGCTGTCCATGTTAAG(+ACTCAC)GTACCTGAGGCAGGAGCTCAGAGCTACTCAC NGG 10 8 17 8 24 0 100 5 10
    240605000205_eulo AGGTACTTCCTAAAGCTGCCCTGCCTTTACAG(+ACTCAC)GATCCCCCGAAAGAGAATTTAGGACGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605000203_31oe CAGGCTGCCTCGTCCTAAATTCTCTTTCGGGG(+ACTCAC)GATCCTGTAAAGGCAGGGCAGCTTTAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605000202_umhb ATGGGGCTGCAGGACGGATGGCCTGTCTCTAG(+ACTCAC)GATCCCACTTTCAAGGCAACAGTCTGGCCAA NGG 10 8 17 8 24 0 100 5 10
    240605000200_daqj AGGCCTTGGCCAGACTGTTGCCTTGAAAGTGG(+ACTCAC)GATCCTAGAGACAGGCCATCCGTCCTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605000158_kwdt TACATTTTCACCTCCAGTCAGCAATTTCCTAG(+ACTCAC)GTACCATGTAAAGCTGTAAAATTGAATTATT NGG 10 8 17 8 24 0 100 5 10