Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000105_l6pf GAGATGCAGCCTCTGAAAAGGGTTCTGTGACG(+ACTCAC)GATCCAAGTCAGTCTTCTGACCCTGGTGCCA NGG 10 8 17 8 24 0 100 5 10
    240605000104_jpwn GATAGGAGGCTATGAGGCCTCCTTAGGTCAGG(+ACTCAC)GATCCCAGCAAGAGGCTGGCTCGAGTTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605000102_iuhc TCTCTCCACAACTCGAGCCAGCCTCTTGCTGG(+ACTCAC)GATCCCTGACCTAAGGAGGCCTCATAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605000059_7vu5 TCAGCTCTTTGTTTGCTCTTTAAACAGCCCAG(+ACTCAC)GATCCTCCGCCTAGGGGAGGAATGGTGCCAA NGG 10 8 17 8 24 0 100 5 10
    240605000058_w3v3 ATAAGTTGGCACCATTCCTCCCCTAGGCGGAG(+ACTCAC)GATCCTGGGCTGTTTAAAGAGCAAACAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605000056_2anm AGTTCAAGCGCAGGGTCCTCCAGCCTGCATAG(+ACTCAC)GATCCTCCAGCACTCCCATCCACTTAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240605000055_j0f6 ACATTGACTCTAAGTGGATGGGAGTGCTGGAG(+ACTCAC)GATCCTATGCAGGCTGGAGGACCCTGCGCTT NGG 10 8 17 8 24 0 100 5 10
    240605000053_6hte AGCTTCTACCCATTCTCTCTCCCACTTCTGAG(+ACTCAC)GATCCCCACAGCTAACATGCAATGCCTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605000051_tjdw GTAGGGAGCAGGCATTGCATGTTAGCTGTGGG(+ACTCAC)GATCCTCAGAAGTGGGAGAGAGAATGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240605000049_qbxx TTTACACTGTAGTATCTCTGTGATCACTGCTG(+ACTCAC)GATCCTCTGTATTCCGAAACAGGTACAAAAC NGG 10 8 17 8 24 0 100 5 10