Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000032_g53j GGCTAGTGGGGGGTAAGAGGCCCCCATCCCAG(+ACTCAC)GATCCATAGCACAAAGCCACGGGCTCCATAA NGG 10 8 17 8 24 0 100 5 10
    240605000030_uss5 CAGACTGGGTAAGATGTGGCTCTCTCAGGTGG(+ACTCAC)GATCCCTTTATTTATTTACTCAAGTCCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605000029_vvge TTTGTATGGGGACTTGAGTAAATAAATAAAGG(+ACTCAC)GATCCCACCTGAGAGAGCCACATCTTACCCA NGG 10 8 17 8 24 0 100 5 10
    240605000027_xs8c TTATTATTCATTTAACCATCCATCCACACAAG(+ACTCAC)GTACCTTCTTGAAACCTCTGGGCAGTCTCAC NGG 10 8 17 8 24 0 100 5 10
    240605000026_cm9m CAAATGTGAGACTGCCCAGAGGTTTCAAGAAG(+ACTCAC)GTACCTTGTGTGGATGGATGGTTAAATGAAT NGG 10 8 17 8 24 0 100 5 10
    240605000024_m046 AAGGAACATTTAACATCAAGATTTAAACAGTG(+ACTCAC)GTACCACTTGGGTGGCAAACTGGTTCTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605000023_xkn9 AGATCTTTAAGAACCAGTTTGCCACCCAAGTG(+ACTCAC)GTACCACTGTTTAAATCTTGATGTTAAATGT NGG 10 8 17 8 24 0 100 5 10
    240605000021_jbxe AAACATTCCATGTTCCATTGTCGCTCCTCCGG(+ACTCAC)GTACCCAGTTGGGACCCTGGTGCCAGCATTT NGG 10 8 17 8 24 0 100 5 10
    240605000019_stlg CACTGAAATGCTGGCACCAGGGTCCCAACTGG(+ACTCAC)GTACCCGGAGGAGCGACAATGGAACATGGAA NGG 10 8 17 8 24 0 100 5 10
    240605000017_v81n CAGTCATGCCCAGCTTTCATAGGGGTTCTGGG(+ACTCAC)GATCCAAACTTAGGTCTTCACACATGCTCAC NGG 10 8 17 8 24 0 100 5 10