Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000048_btk9 TGTCTGTTTTGTACCTGTTTCGGAATACAGAG(+ACTCAC)GATCCAGCAGTGATCACAGAGATACTACAGT NGG 10 8 17 8 24 0 100 5 10
    240605000047_j19z GCCTGAGATTCCATTTCTTCTTCTCTTCACAG(+ACTCAC)GTACCTGAATTCAGGTGTGTCTCTTGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605000046_ww4i AATCTCAGGGCAAGAGACACACCTGAATTCAG(+ACTCAC)GTACCTGTGAAGAGAAGAAGAAATGGAATCT NGG 10 8 17 8 24 0 100 5 10
    240605000044_xa2a GCAGAAAGCCTATGTCCTGCATGCTGGAACAG(+ACTCAC)GTACCTGTCTGTGTGTGGCCTTTCCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240605000043_b295 CCCAGATGTGGGGAAAGGCCACACACAGACAG(+ACTCAC)GTACCTGTTCCAGCATGCAGGACATAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605000041_jdpx CCCATTTCCGTTCACAGTATGTTGGGGCCCAG(+ACTCAC)GTACCAACAGTGTAGGGCTTTCTGATACCCT NGG 10 8 17 8 24 0 100 5 10
    240605000040_5a2n TCCTGAGGGTATCAGAAAGCCCTACACTGTTG(+ACTCAC)GTACCTGGGCCCCAACATACTGTGAACGGAA NGG 10 8 17 8 24 0 100 5 10
    240605000038_b2ww GGGGAGGGAGATGAGGCTGCCCCAAGGCTCTG(+ACTCAC)GTACCTGGCTTTAGTTTTCTGTCTTCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605000036_4q0v CCTGAGGAAAGAAGACAGAAAACTAAAGCCAG(+ACTCAC)GTACCAGAGCCTTGGGGCAGCCTCATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605000035_zo7c GAGTATTATGGAGCCCGTGGCTTTGTGCTATG(+ACTCAC)GATCCTGGGATGGGGGCCTCTTACCCCCCAC NGG 10 8 17 8 24 0 100 5 10