Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235942_qccy AAGCCTAGAGGTCCCTTTGAAGACATCCTCAG(+ACTCAC)GATCCCATATCAGTTCTCTACTGTATCGTGA NGG 10 8 17 8 24 0 100 5 10
    240604235941_om4w AGTTATCACGATACAGTAGAGAACTGATATGG(+ACTCAC)GATCCTGAGGATGTCTTCAAAGGGACCTCTA NGG 10 8 17 8 24 0 100 5 10
    240604235939_3z07 AAGCCTAGAGGTCCCTTTGAAGACATCCTCAG(+ACTCAC)GATCCCATATCAGTTCTCTACTGTATCGTGA NGG 10 8 17 8 24 0 100 5 10
    240604235936_yxt3 GAGTTCACAGCTGAGGTCTCATGCGACCCCAG(+ACTCAC)GATCCCCCAGCCAATGATAAAGCACAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240604235934_s267 GTTACTGCACTGTGCTTTATCATTGGCTGGGG(+ACTCAC)GATCCTGGGGTCGCATGAGACCTCAGCTGTG NGG 10 8 17 8 24 0 100 5 10
    240604235933_k9j7 ACCTGCATCTTTGTTCCCTAAATTAGTGCTTG(+ACTCAC)GTACCCAGGAAGATCAATTAGTATGTACTGA NGG 10 8 17 8 24 0 100 5 10
    240604235930_5iby TTAACTCAGTACATACTAATTGATCTTCCTGG(+ACTCAC)GTACCAAGCACTAATTTAGGGAACAAAGATG NGG 10 8 17 8 24 0 100 5 10
    240604235929_hfpi AACTGTACAATCCCAAGATAACTTGAATATAG(+ACTCAC)GTACCAAAGTTACATAAAAATTCAGTCAAAA NGG 10 8 17 8 24 0 100 5 10
    240604235928_9qc4 TTACATTTTGACTGAATTTTTATGTAACTTTG(+ACTCAC)GTACCTATATTCAAGTTATCTTGGGATTGTA NGG 10 8 17 8 24 0 100 5 10
    240604235926_iciq GGGAATCCAGCACCCTTTTCTGGACTCCATGG(+ACTCAC)GTACCTGCAATCACACATGATGTGCATGCAC NGG 10 8 17 8 24 0 100 5 10