Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235924_fz80 TGTCTGTGCATGCACATCATGTGTGATTGCAG(+ACTCAC)GTACCCATGGAGTCCAGAAAAGGGTGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240604235923_hjhb TATTTGTGTGTGGAGAGAGAAAGAGACTGAAG(+ACTCAC)GATCCCCTTCCCTTTTTTTCTTTGTGCTAGG NGG 10 8 17 8 24 0 100 5 10
    240604235921_croy GAGGTCCTAGCACAAAGAAAAAAAGGGAAGGG(+ACTCAC)GATCCTTCAGTCTCTTTCTCTCTCCACACAC NGG 10 8 17 8 24 0 100 5 10
    240604235920_bz2n AAGTAGGGTGAGGCCTTAAGCTTTCAAATCTG(+ACTCAC)GATCCCAGGGAAGCACTTCCTTTAACAAGGC NGG 10 8 17 8 24 0 100 5 10
    240604235917_ti3p TGGTGGCCTTGTTAAAGGAAGTGCTTCCCTGG(+ACTCAC)GATCCAGATTTGAAAGCTTAAGGCCTCACCC NGG 10 8 17 8 24 0 100 5 10
    240604235915_03wp GCCACATGGAAATCTTTAATTTATTTGTTGAG(+ACTCAC)GTACCATATAGCCAGGGTGCTAGGTGATGAG NGG 10 8 17 8 24 0 100 5 10
    240604235913_5vpp CACCACTCATCACCTAGCACCCTGGCTATATG(+ACTCAC)GTACCTCAACAAATAAATTAAAGATTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240604235912_rl6h AACTACTGGTTTGCTGTGTAAATGGATGTCAG(+ACTCAC)GATCCAGCAAAACTACTGCTTTCTTCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240604235911_p35y TGTCTTTGGAGAAGAAAGCAGTAGTTTTGCTG(+ACTCAC)GATCCTGACATCCATTTACACAGCAAACCAG NGG 10 8 17 8 24 0 100 5 10
    240604235909_yn7l CCTACTAGGTTCTAACTGATGCAGAGTGAAGG(+ACTCAC)GTACCGAGAAAGTACATGTACATAAAGATTT NGG 10 8 17 8 24 0 100 5 10