Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235839_qlsn GTGCTTCTTAGGCCTTAGGCCTGTGAGATCAG(+ACTCAC)GTACCTTGCCTGCGTGTCTGTTTTTCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240604235836_xuqm TATTTGGCTGTAGCTCTGATCAACTTTCCCTG(+ACTCAC)GTACCCTCATCCTACCTTCATATACAGGTAG NGG 10 8 17 8 24 0 100 5 10
    240604235834_5vke TGGAGCTACCTGTATATGAAGGTAGGATGAGG(+ACTCAC)GTACCAGGGAAAGTTGATCAGAGCTACAGCC NGG 10 8 17 8 24 0 100 5 10
    240604235832_h1lo CATCCCAAAGTTGGCCTTTGTCCTTCCCCATG(+ACTCAC)GATCCCTAAAAACAAACAAACAAATAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240604235830_yaoy TTTATTTTTTTATTTGTTTGTTTGTTTTTAGG(+ACTCAC)GATCCATGGGGAAGGACAAAGGCCAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240604235829_6gz0 AGGTTCCAGACTTCTCCCAGCCACCGTGGCAG(+ACTCAC)GTACCCCTCATGTCAGGGACTGCCTCGTGCT NGG 10 8 17 8 24 0 100 5 10
    240604235827_kge7 AAAGCAGCACGAGGCAGTCCCTGACATGAGGG(+ACTCAC)GTACCTGCCACGGTGGCTGGGAGAAGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240604235826_u7at TAGTTAGCGATGAGGAGGAAGACACTTGAATG(+ACTCAC)GATCCCCACCCTAGGATGCTGCTCTTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240604235824_2wx7 GGAAGGCAAGAAGAGCAGCATCCTAGGGTGGG(+ACTCAC)GATCCATTCAAGTGTCTTCCTCCTCATCGCT NGG 10 8 17 8 24 0 100 5 10
    240604235822_0n9f TATTGATTCTCTTAGAACTTTGTCAGTGGATG(+ACTCAC)GTACCTTGCCTTTGCAGGTTGTATGTGACCT NGG 10 8 17 8 24 0 100 5 10