Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235738_fues AGACATTTTAGGGAGATAGTGCCAGCTGAAGG(+ACTCAC)GATCCTGTAGATTTGAAGCTATCAACTTATT NGG 10 8 17 8 24 0 100 5 10
    240604235737_vl36 TTACAGGGAAATTCATTATAGATATTCTTTTG(+ACTCAC)GTACCTGTGAATTAAATATAAATATAAGATG NGG 10 8 17 8 24 0 100 5 10
    240604235735_zi5n GAGTTCATCTTATATTTATATTTAATTCACAG(+ACTCAC)GTACCAAAAGAATATCTATAATGAATTTCCC NGG 10 8 17 8 24 0 100 5 10
    240604235734_ux5w ATACCCCTTGGCTATGCCCACAAATCCATAAG(+ACTCAC)GATCCTGACTTAGAAAGAAAAACAGCACAAA NGG 10 8 17 8 24 0 100 5 10
    240604235733_r34z TTGAATTTGTGCTGTTTTTCTTTCTAAGTCAG(+ACTCAC)GATCCTTATGGATTTGTGGGCATAGCCAAGG NGG 10 8 17 8 24 0 100 5 10
    240604235731_4fji AGCAAGGCTCTCTCTTCTGGGCAAGGGCAATG(+ACTCAC)GATCCTCAGAGGCCCCGGCAGAAGCCTAGCT NGG 10 8 17 8 24 0 100 5 10
    240604235730_oppm AGAGAAGCTAGGCTTCTGCCGGGGCCTCTGAG(+ACTCAC)GATCCATTGCCCTTGCCCAGAAGAGAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240604235729_lo1d GTCTTCACACTCTCCTGTATGTATCTGTATTG(+ACTCAC)GTACCAATGAAATACAGTTCAGCATTTAATA NGG 10 8 17 8 24 0 100 5 10
    240604235728_jt1l TAGCATATTAAATGCTGAACTGTATTTCATTG(+ACTCAC)GTACCAATACAGATACATACAGGAGAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240604235727_tc43 TCTCCCAACTATTCATAATTCTAGTTCAAGTG(+ACTCAC)GATCCAGCACCCTCTTCTAACTGATACTGCC NGG 10 8 17 8 24 0 100 5 10