Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235725_djjh GTACTGGCAGTATCAGTTAGAAGAGGGTGCTG(+ACTCAC)GATCCACTTGAACTAGAATTATGAATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240604235722_ra8w TTTGTAGACCAGGCTGGCCTTGAAGTCCCAGG(+ACTCAC)GATCCTGTCTGTGCCTCTCACATGTGTTAAG NGG 10 8 17 8 24 0 100 5 10
    240604235721_f635 TTAGTCTTAACACATGTGAGAGGCACAGACAG(+ACTCAC)GATCCCTGGGACTTCAAGGCCAGCCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240604235719_9pdw TCCTGGCAGGTGATCTACAAAGCAGAAGCGTG(+ACTCAC)GTACCCAGTCAGTCACAGAGAATAGCATCCT NGG 10 8 17 8 24 0 100 5 10
    240604235718_kfe6 AATCAAGGATGCTATTCTCTGTGACTGACTGG(+ACTCAC)GTACCACGCTTCTGCTTTGTAGATCACCTGC NGG 10 8 17 8 24 0 100 5 10
    240604235716_64lj CACACTGTAAGAGGTCCCTAGTCTGTGTGGTG(+ACTCAC)GTACCTTTAATGCCAGCACTTGGGAGGCAGA NGG 10 8 17 8 24 0 100 5 10
    240604235715_vq6z CTGCTTCTGCCTCCCAAGTGCTGGCATTAAAG(+ACTCAC)GTACCACCACACAGACTAGGGACCTCTTACA NGG 10 8 17 8 24 0 100 5 10
    240604235713_yp6u TAAGAGTCGGCAGCCTGGCAGCGGTACTGGGG(+ACTCAC)GATCCGTGCCCAGGCAGCTGTCAAGCATTCC NGG 10 8 17 8 24 0 100 5 10
    240604235711_4bpx GGGAGGGAATGCTTGACAGCTGCCTGGGCACG(+ACTCAC)GATCCCCCAGTACCGCTGCCAGGCTGCCGAC NGG 10 8 17 8 24 0 100 5 10
    240604235710_17z2 TAGCTTTGTTACATCCTAGCAAGATATTCCTG(+ACTCAC)GATCCCTGCTGCGCATTCTGATGTGAATCCC NGG 10 8 17 8 24 0 100 5 10