Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235636_gtd1 GGTGGTTCCTTTGGAGCCGTGAACATTCCATG(+ACTCAC)GTACCTGGCACTGCTTTAGAAAGCAAATGAC NGG 10 8 17 8 24 0 100 5 10
    240604235634_zzuz ACAAAGGATGAGCCCTGCTGTGTTGCAAGTAG(+ACTCAC)GATCCCCTCCCCCAGGTCTATGAGGCTAATA NGG 10 8 17 8 24 0 100 5 10
    240604235632_8h9a AATCTTATTAGCCTCATAGACCTGGGGGAGGG(+ACTCAC)GATCCTACTTGCAACACAGCAGGGCTCATCC NGG 10 8 17 8 24 0 100 5 10
    240604235631_m2o1 AACAGATGAAATCTTTCATGGCTGAAATCTTG(+ACTCAC)GATCCCTAAATGACCCGCCACCGTGGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240604235630_lgo0 TTATAAAAGCCCACGGTGGCGGGTCATTTAGG(+ACTCAC)GATCCAAGATTTCAGCCATGAAAGATTTCAT NGG 10 8 17 8 24 0 100 5 10
    240604235628_43we AGAGCATCCCTCTGGAGGTAAGGTCCTCTCAG(+ACTCAC)GTACCTTCTCACACCATCCCCAGTTACACCA NGG 10 8 17 8 24 0 100 5 10
    240604235627_1snb GTTTCTGGTGTAACTGGGGATGGTGTGAGAAG(+ACTCAC)GTACCTGAGAGGACCTTACCTCCAGAGGGAT NGG 10 8 17 8 24 0 100 5 10
    240604235626_h2sw AGAAAATCTTGCAAAATACATGGTTGCTTCAG(+ACTCAC)GTACCAGCTCTTTACAATACAGTTCAAACTC NGG 10 8 17 8 24 0 100 5 10
    240604235624_5g4j AAAAAGAGTTTGAACTGTATTGTAAAGAGCTG(+ACTCAC)GTACCTGAAGCAACCATGTATTTTGCAAGAT NGG 10 8 17 8 24 0 100 5 10
    240604235623_h1s3 CCAAGCTTTTTTTTTTTTAAGGCAAATTTTTG(+ACTCAC)GTACCTTTTTTCCTAAACATCTTCGTGAATA NGG 10 8 17 8 24 0 100 5 10