Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235606_lo23 GAAAATAAAAAATATTTTTTCTATCTAAATTG(+ACTCAC)GATCCAAGCTCCATTATCAGTTCTCAAGGTA NGG 10 8 17 8 24 0 100 5 10
    240604235605_sjur TGTATGTAACCCATGTCAATCAGATGCAGGTG(+ACTCAC)GTACCCACACGACCAGAGTGATCAATAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604235603_eh5z GAAAATTCCTATTGATCACTCTGGTCGTGTGG(+ACTCAC)GTACCACCTGCATCTGATTGACATGGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240604235601_emel CCACAAGGCACGATATTTATATCCACCTAGGG(+ACTCAC)GTACCGGCTTTTACTATTTAAAGCTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240604235559_sql1 GTTTCAAAAATAGCTTTAAATAGTAAAAGCCG(+ACTCAC)GTACCCCTAGGTGGATATAAATATCGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240604235557_8h28 TGGCTCTGCAGCCACATCGTGGCTATGTTCCG(+ACTCAC)GATCCCATCCAGGGTGTGCATGTGCCAAGAA NGG 10 8 17 8 24 0 100 5 10
    240604235556_kwaz CGCATTTCTTGGCACATGCACACCCTGGATGG(+ACTCAC)GATCCGGAACATAGCCACGATGTGGCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240604235554_8w4p GGACATTAACCTTTGGAAGTCTATATACAAAG(+ACTCAC)GTACCATGGAGTAACTGACAGCTACCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240604235552_0kik AAGAGAACTTGGTAGCTGTCAGTTACTCCATG(+ACTCAC)GTACCTTTGTATATAGACTTCCAAAGGTTAA NGG 10 8 17 8 24 0 100 5 10
    240604235550_wz31 CATGGTGGGTAGGTACGCATCGTGATGCATGG(+ACTCAC)GATCCACTAATTGTGACTTGAGAAGTTATCA NGG 10 8 17 8 24 0 100 5 10