Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235535_22rh CTGAAGCTCGGGGTGGGGAGGGCGGGTGTCAG(+ACTCAC)GATCCAGACTTACTCGAAGCCATCTGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240604235533_dee9 CTGGTAAACTTGCCTTCCCGTTTCTCTGCCTG(+ACTCAC)GTACCTTATTTGAGCAGGCTGTCAGCAATGG NGG 10 8 17 8 24 0 100 5 10
    240604235532_wxu7 AAAGGCCATTGCTGACAGCCTGCTCAAATAAG(+ACTCAC)GTACCAGGCAGAGAAACGGGAAGGCAAGTTT NGG 10 8 17 8 24 0 100 5 10
    240604235530_bglm CTTGCAAAGTGCTCAGGAACACAGAATGTCTG(+ACTCAC)GATCCTGATGTCAAGCAGGGTCCGGGAGCTT NGG 10 8 17 8 24 0 100 5 10
    240604235529_uf78 TGTGCAAGCTCCCGGACCCTGCTTGACATCAG(+ACTCAC)GATCCAGACATTCTGTGTTCCTGAGCACTTT NGG 10 8 17 8 24 0 100 5 10
    240604235528_ekp1 TTCCTACTTGAAGCCCTTGCCCGTTCCAGCTG(+ACTCAC)GATCCTTGGTGAGGACTACCGAGGTTTAACC NGG 10 8 17 8 24 0 100 5 10
    240604235525_8jz1 GTGGAGGTTAAACCTCGGTAGTCCTCACCAAG(+ACTCAC)GATCCAGCTGGAACGGGCAAGGGCTTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240604235524_57d4 GTGCTCATCACCCACATGGCCAATCCAGATGG(+ACTCAC)GTACCTAGTTATTCTGGCTCCCTGAGGGTCT NGG 10 8 17 8 24 0 100 5 10
    240604235522_g2ip TCCAGAGACCCTCAGGGAGCCAGAATAACTAG(+ACTCAC)GTACCCATCTGGATTGGCCATGTGGGTGATG NGG 10 8 17 8 24 0 100 5 10
    240604235521_94y2 GCTGGAACTCCCTCTATAGCCTGAGACTCAGG(+ACTCAC)GATCCACCTGGCTTTGCCTCCTCAGTGCTGA NGG 10 8 17 8 24 0 100 5 10