Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235519_iigj TGGTCTCAGCACTGAGGAGGCAAAGCCAGGTG(+ACTCAC)GATCCCTGAGTCTCAGGCTATAGAGGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240604235518_x8i2 GATCCACATGGAAGGCTCCAACAGCAACAAGG(+ACTCAC)GATCCATGCAGCCTGCAGAGAGCTTTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240604235516_37yd GAAAAGTAGAAAAGCTCTCTGCAGGCTGCATG(+ACTCAC)GATCCCTTGTTGCTGTTGGAGCCTTCCATGT NGG 10 8 17 8 24 0 100 5 10
    240604235515_sjxh TGGGATGTTTTTTAGGTCTTTGCTATTGCAGG(+ACTCAC)GTACCATCCCTTTGAAGCCCTTTCCTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240604235513_c22o CAAAAACAGGAGGAAAGGGCTTCAAAGGGATG(+ACTCAC)GTACCCTGCAATAGCAAAGACCTAAAAAACA NGG 10 8 17 8 24 0 100 5 10
    240604235511_3xu1 TCAAGGTCAAAGAAACATAGAAGGGTAAGAGG(+ACTCAC)GTACCCTGTGAAATGATCCTTTCATGCACCT NGG 10 8 17 8 24 0 100 5 10
    240604235509_y81i AGAGAAGGTGCATGAAAGGATCATTTCACAGG(+ACTCAC)GTACCCTCTTACCCTTCTATGTTTCTTTGAC NGG 10 8 17 8 24 0 100 5 10
    240604235508_2kk5 AGTGACAGATTAACAGGGGTAGCTGCTACTTG(+ACTCAC)GATCCACAGAATCTCTGCAAGTGAGGGTTAA NGG 10 8 17 8 24 0 100 5 10
    240604235507_o6ch CATAGTTAACCCTCACTTGCAGAGATTCTGTG(+ACTCAC)GATCCAAGTAGCAGCTACCCCTGTTAATCTG NGG 10 8 17 8 24 0 100 5 10
    240604235506_mhew CAGCCCCAGCAAGCGCCCAGGAACAGGGAGAG(+ACTCAC)GATCCATGCATGTGAGCAGCCAGCATCAAGG NGG 10 8 17 8 24 0 100 5 10