Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235622_ahbn TTTAATATTCACGAAGATGTTTAGGAAAAAAG(+ACTCAC)GTACCAAAAATTTGCCTTAAAAAAAAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240604235620_woj1 ATGAACACACACATAATCCAGCACGTAGTAGG(+ACTCAC)GATCCTCCTCGCCTAGGCTAAATCACTTTAG NGG 10 8 17 8 24 0 100 5 10
    240604235619_c683 TTCACCTAAAGTGATTTAGCCTAGGCGAGGAG(+ACTCAC)GATCCCTACTACGTGCTGGATTATGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240604235617_bv7h CCTACATAAGCCCTTCATTGTGGGAGTAAATG(+ACTCAC)GTACCGAAGCCCCAGAAAGTCCTACAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240604235615_dtuf AAAAAATCTCTGTAGGACTTTCTGGGGCTTCG(+ACTCAC)GTACCATTTACTCCCACAATGAAGGGCTTAT NGG 10 8 17 8 24 0 100 5 10
    240604235614_rh7l TGTGATCCCAACACAGGAAGTGGCAACAGGAG(+ACTCAC)GATCCAGAGTTCAAAGTCAGCCTTGGATACA NGG 10 8 17 8 24 0 100 5 10
    240604235613_bnef GCATATGTATCCAAGGCTGACTTTGAACTCTG(+ACTCAC)GATCCTCCTGTTGCCACTTCCTGTGTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240604235611_zetp GCAATCGAGACAGAGAGAGCCAGCAAGGGCTG(+ACTCAC)GTACCTGAGGTCATATTTCAGACACCCACCT NGG 10 8 17 8 24 0 100 5 10
    240604235609_3dng GGGTCAGGTGGGTGTCTGAAATATGACCTCAG(+ACTCAC)GTACCAGCCCTTGCTGGCTCTCTCTGTCTCG NGG 10 8 17 8 24 0 100 5 10
    240604235608_ru52 GCTACTACCTTGAGAACTGATAATGGAGCTTG(+ACTCAC)GATCCAATTTAGATAGAAAAAATATTTTTTA NGG 10 8 17 8 24 0 100 5 10