Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235651_201s GCCCTGAAGTTGTGTTTCCCCCCCCCCCTCTG(+ACTCAC)GATCCTAACTCTCCATTTGCCAAGTTTGTTA NGG 10 8 17 8 24 0 100 5 10
    240604235650_sh3h ACACATGATTAAAAATAGATTTAAAAGTTATG(+ACTCAC)GTACCTTAATCAAAGTTTCTGATACATAATG NGG 10 8 17 8 24 0 100 5 10
    240604235649_wv1o AAATTCATTATGTATCAGAAACTTTGATTAAG(+ACTCAC)GTACCATAACTTTTAAATCTATTTTTAATCA NGG 10 8 17 8 24 0 100 5 10
    240604235648_xfxd AGGTTCATGGATCTATCAGAGGCTCCAGATCG(+ACTCAC)GTACCATGTGTAGGAGCCCTGAGCCTGAAAC NGG 10 8 17 8 24 0 100 5 10
    240604235647_roym GTTCAGTTTCAGGCTCAGGGCTCCTACACATG(+ACTCAC)GTACCGATCTGGAGCCTCTGATAGATCCATG NGG 10 8 17 8 24 0 100 5 10
    240604235645_ax3h CCTATTGCATTCCTCATTGCTCTGAGCTTTAG(+ACTCAC)GATCCCCTGCCCTGGAACCCTGGCAGCTCAA NGG 10 8 17 8 24 0 100 5 10
    240604235643_f14n GGTTCTTGAGCTGCCAGGGTTCCAGGGCAGGG(+ACTCAC)GATCCTAAAGCTCAGAGCAATGAGGAATGCA NGG 10 8 17 8 24 0 100 5 10
    240604235641_mn89 GGGTGAGGGGAGGTCCCTGCCACAAAACAAAG(+ACTCAC)GTACCAGGAACAAAACCGAGCCTTCAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240604235639_yrn8 TTGCAATTCCTGAAGGCTCGGTTTTGTTCCTG(+ACTCAC)GTACCTTTGTTTTGTGGCAGGGACCTCCCCT NGG 10 8 17 8 24 0 100 5 10
    240604235637_avcy GTTCTGTCATTTGCTTTCTAAAGCAGTGCCAG(+ACTCAC)GTACCATGGAATGTTCACGGCTCCAAAGGAA NGG 10 8 17 8 24 0 100 5 10