Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235753_uvlq CATTATGTTGTACCCTACCTCCGAAATCACAG(+ACTCAC)GTACCATATGATTCCAGTAGATTTTAGCTAA NGG 10 8 17 8 24 0 100 5 10
    240604235751_rlfc CGTACAAAGTGGGCGGTGCTGAGGGGCTTGGG(+ACTCAC)GATCCTTGCTGCAAAGCTTTGCAGAATGTAA NGG 10 8 17 8 24 0 100 5 10
    240604235750_hniu GCCTCTTACATTCTGCAAAGCTTTGCAGCAAG(+ACTCAC)GATCCCCAAGCCCCTCAGCACCGCCCACTTT NGG 10 8 17 8 24 0 100 5 10
    240604235748_6rz6 CCCTTCAGACTGAGTAAACTTTTCAATATATG(+ACTCAC)GTACCCAGATGTTTTATTTATGAAGTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240604235747_6bn6 TAAAATAAAAACTTCATAAATAAAACATCTGG(+ACTCAC)GTACCATATATTGAAAAGTTTACTCAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240604235745_wxy9 AAACAAACCAAACAAAACATAAAAAGCCTTTG(+ACTCAC)GTACCTTTCAATAACAAAAGAAAAATATATT NGG 10 8 17 8 24 0 100 5 10
    240604235743_cm9p ATCTAAATATATTTTTCTTTTGTTATTGAAAG(+ACTCAC)GTACCAAAGGCTTTTTATGTTTTGTTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240604235742_axwe GAGGCAACTGCAGACGAATCAGAAAATAAGAG(+ACTCAC)GATCCACCACCACAGAACTGATTGGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240604235741_o98r ATTGCTCTGAGCCAATCAGTTCTGTGGTGGTG(+ACTCAC)GATCCTCTTATTTTCTGATTCGTCTGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240604235739_ikpu GTAAGAATAAGTTGATAGCTTCAAATCTACAG(+ACTCAC)GATCCCTTCAGCTGGCACTATCTCCCTAAAA NGG 10 8 17 8 24 0 100 5 10