Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235432_be97 GCCAAATGAACTGGAGGGAATCAGGATGCTGG(+ACTCAC)GTACCTTCCAAACATCAGAAAGCAGCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240604235430_3ozw GTCGGGCCCCCAACAACTTGAAGCTTCCTCTG(+ACTCAC)GATCCTCACTTGAGCTCTTTGGGGATGGGGC NGG 10 8 17 8 24 0 100 5 10
    240604235429_k8eq CCTAGGCCCCATCCCCAAAGAGCTCAAGTGAG(+ACTCAC)GATCCAGAGGAAGCTTCAAGTTGTTGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240604235428_36ne CTATGCTACCCCACTTCCTGTATGCTCAACTG(+ACTCAC)GATCCTCAAGACCATTTCATCTCTAGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240604235426_hyr5 CGTGGTGCAGCTAGAGATGAAATGGTCTTGAG(+ACTCAC)GATCCAGTTGAGCATACAGGAAGTGGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240604235425_mlq3 TGACAGACTTGATGCTTTGAAATTGACCATTG(+ACTCAC)GTACCTCCTCCTTAAAAGAGAAGTGTCGTGT NGG 10 8 17 8 24 0 100 5 10
    240604235423_2rqd CCTGAACACGACACTTCTCTTTTAAGGAGGAG(+ACTCAC)GTACCAATGGTCAATTTCAAAGCATCAAGTC NGG 10 8 17 8 24 0 100 5 10
    240604235422_p3k4 CTGGGTGTGCAGGAATTCTGCCTGTGAAGTGG(+ACTCAC)GATCCACAGCATGTTCGACGGGTGGGAGAAC NGG 10 8 17 8 24 0 100 5 10
    240604235420_6heo GCCCAGTTCTCCCACCCGTCGAACATGCTGTG(+ACTCAC)GATCCCACTTCACAGGCAGAATTCCTGCACA NGG 10 8 17 8 24 0 100 5 10
    240604235419_cw8y CTTGTTCTACCATCTCTCCTGGGTTCTTCATG(+ACTCAC)GTACCACCTCGTGTACAGCCACGCCAGTTAA NGG 10 8 17 8 24 0 100 5 10