Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235323_081i TCATCCCCAGCTCCAAATCCATTGATCCAGAG(+ACTCAC)GATCCAATGCCCTCTTCTGACTTCTATGGGC NGG 10 8 17 8 24 0 100 5 10
    240604235322_iomw GTTGACTGAGATGGCAGCCAGTGTGCTGAGTG(+ACTCAC)GATCCAAACATCACAATGGTGTGACCACTTG NGG 10 8 17 8 24 0 100 5 10
    240604235320_yl5e CCTTTCAAGTGGTCACACCATTGTGATGTTTG(+ACTCAC)GATCCACTCAGCACACTGGCTGCCATCTCAG NGG 10 8 17 8 24 0 100 5 10
    240604235319_dtnm TCATGTGTCTTGGAACCCAGTTACTCTAAGAG(+ACTCAC)GTACCTGGTACAATGTGTATGACTCACGTGC NGG 10 8 17 8 24 0 100 5 10
    240604235317_6fxz AAGAAGCACGTGAGTCATACACATTGTACCAG(+ACTCAC)GTACCTCTTAGAGTAACTGGGTTCCAAGACA NGG 10 8 17 8 24 0 100 5 10
    240604235316_o9m7 CGGGCAGAGACTTGCTGAACTTTTAACTTGAG(+ACTCAC)GATCCTTCTCTTGAGAAAACCTGTGGGCATT NGG 10 8 17 8 24 0 100 5 10
    240604235315_nlev GCTCCAATGCCCACAGGTTTTCTCAAGAGAAG(+ACTCAC)GATCCTCAAGTTAAAAGTTCAGCAAGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240604235313_h7km CAGAGTATATATTATGTACACACATACATATG(+ACTCAC)GATCCATACACGTAAGTGCACATACGTACAC NGG 10 8 17 8 24 0 100 5 10
    240604235312_1u5e TGTGTGTGTACGTATGTGCACTTACGTGTATG(+ACTCAC)GATCCATATGTATGTGTGTACATAATATATA NGG 10 8 17 8 24 0 100 5 10
    240604235310_bqim AAGGACCATTGCATGTCATAAGACCTGCCCTG(+ACTCAC)GATCCCTTTAAAAGTCTTTTCACAGGACACT NGG 10 8 17 8 24 0 100 5 10