Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235401_n9b2 GCCCCAGCCCTCGTACCACCCTGTTACCCCAG(+ACTCAC)GATCCATCTGCCCTTGTAAAAGGACTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240604235359_hj4y AACCCCAGCACTTGTGTTACCTCAGGGCCTTG(+ACTCAC)GTACCTGGATACTGCCACAGAATTTCCATGG NGG 10 8 17 8 24 0 100 5 10
    240604235357_a3qw CCGCCCCATGGAAATTCTGTGGCAGTATCCAG(+ACTCAC)GTACCAAGGCCCTGAGGTAACACAAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240604235355_tzhk TTACCAGCCCTCAACCTCAGTTCTGAGGGTGG(+ACTCAC)GATCCCATCGCTCAGCAAGGCTTCATCCTGA NGG 10 8 17 8 24 0 100 5 10
    240604235353_6cbp GGAGGTCAGGATGAAGCCTTGCTGAGCGATGG(+ACTCAC)GATCCCACCCTCAGAACTGAGGTTGAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240604235351_ntyk CAGCACGCCTGGCCTGCCCACCTCCACCTTGG(+ACTCAC)GTACCTCGTAGCCTAGGGACGGCAGGACTTT NGG 10 8 17 8 24 0 100 5 10
    240604235350_ejam GTGTTAAAGTCCTGCCGTCCCTAGGCTACGAG(+ACTCAC)GTACCCAAGGTGGAGGTGGGCAGGCCAGGCG NGG 10 8 17 8 24 0 100 5 10
    240604235347_v9wb ATAATAATAGGGTTGGTCCAGCGTTTGCCTGG(+ACTCAC)GATCCCCAGGACAGGTCCCCAGCGTGGAGCG NGG 10 8 17 8 24 0 100 5 10
    240604235345_f8xh CACAGCGCTCCACGCTGGGGACCTGTCCTGGG(+ACTCAC)GATCCCAGGCAAACGCTGGACCAACCCTATT NGG 10 8 17 8 24 0 100 5 10
    240604235343_i62f CTGGAGTGGCCAGTACAGGATCTGGAAAGGAG(+ACTCAC)GTACCACGGGCCATGGCTGGGCAGGCTAAAA NGG 10 8 17 8 24 0 100 5 10