Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235308_5iue GAATTAGTGTCCTGTGAAAAGACTTTTAAAGG(+ACTCAC)GATCCAGGGCAGGTCTTATGACATGCAATGG NGG 10 8 17 8 24 0 100 5 10
    240604235306_xnho TCAATGAGAAATATGGTCCTGCTCTAGTCCTG(+ACTCAC)GATCCAAGAGAGGCAAGAAACCAGCGGCCGC NGG 10 8 17 8 24 0 100 5 10
    240604235305_lha1 GATGAGCGGCCGCTGGTTTCTTGCCTCTCTTG(+ACTCAC)GATCCAGGACTAGAGCAGGACCATATTTCTC NGG 10 8 17 8 24 0 100 5 10
    240604235303_q91s TTGCCTGCATGTCTGTCTGTGTGAGGGTGGAG(+ACTCAC)GATCCCCTGGAGTCATAGAAAGTTGTGAGGT NGG 10 8 17 8 24 0 100 5 10
    240604235300_l70x ATGGCACCTCACAACTTTCTATGACTCCAGGG(+ACTCAC)GATCCTCCACCCTCACACAGACAGACATGCA NGG 10 8 17 8 24 0 100 5 10
    240604235258_k46o TATTTGTAGAGGGTAAAACCTGAGCCTGCCGG(+ACTCAC)GATCCTCCCCCCAACCCCCTGCTTGCCCGGG NGG 10 8 17 8 24 0 100 5 10
    240604235256_8r1d AGGCCCCCGGGCAAGCAGGGGGTTGGGGGGAG(+ACTCAC)GATCCCGGCAGGCTCAGGTTTTACCCTCTAC NGG 10 8 17 8 24 0 100 5 10
    240604235254_nl0f AGGCAGACAGCACCCTGTGGGAGATCAGATGG(+ACTCAC)GATCCTGAGCCCCTTGTATGGCCGTGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240604235253_ey50 AGAGGCCACCCACGGCCATACAAGGGGCTCAG(+ACTCAC)GATCCCATCTGATCTCCCACAGGGTGCTGTC NGG 10 8 17 8 24 0 100 5 10
    240604235250_sbcu AGGGGCCAACAACACAGTGTAAAGAGACCCAG(+ACTCAC)GATCCCTCTGTGATCCCCACCACAATGGAGT NGG 10 8 17 8 24 0 100 5 10