Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235341_80ue AAAGCTTTTAGCCTGCCCAGCCATGGCCCGTG(+ACTCAC)GTACCTCCTTTCCAGATCCTGTACTGGCCAC NGG 10 8 17 8 24 0 100 5 10
    240604235337_6c59 ATAAAGTGTTTCTTACTGTTTGTAACCCCCTG(+ACTCAC)GTACCAGTGTGTCCATCTCCAGGCTCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240604235336_6u0x AGAGGGCAAGGAGCCTGGAGATGGACACACTG(+ACTCAC)GTACCAGGGGGTTACAAACAGTAAGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240604235335_du4x GTAAGAAGTCTCCATGGCTCTGGGTCAGCAGG(+ACTCAC)GTACCAGAGATACTGCCCTTGCCAAATACTT NGG 10 8 17 8 24 0 100 5 10
    240604235334_yc32 CCATCAAGTATTTGGCAAGGGCAGTATCTCTG(+ACTCAC)GTACCCTGCTGACCCAGAGCCATGGAGACTT NGG 10 8 17 8 24 0 100 5 10
    240604235331_w0yd ATAAAGTGTTTCTTACTGTTTGTAACCCCCTG(+ACTCAC)GTACCAGTGTGTCCATCTCCAGGCTCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240604235329_y691 AGAGGGCAAGGAGCCTGGAGATGGACACACTG(+ACTCAC)GTACCAGGGGGTTACAAACAGTAAGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240604235328_61l5 GTGGAAACCTCAGGTTATTGTACTTGTATGAG(+ACTCAC)GTACCCTAGGCAATTCGGCCACTTCTGAAAT NGG 10 8 17 8 24 0 100 5 10
    240604235326_wn2x TCCTGATTTCAGAAGTGGCCGAATTGCCTAGG(+ACTCAC)GTACCTCATACAAGTACAATAACCTGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240604235325_w00n CCAGTGCCCATAGAAGTCAGAAGAGGGCATTG(+ACTCAC)GATCCTCTGGATCAATGGATTTGGAGCTGGG NGG 10 8 17 8 24 0 100 5 10