Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235446_k95o TTACATACTAAAAGAACACATGCCTTTAAGTG(+ACTCAC)GTACCACTTGAGTATAAATTAACTTCTCACT NGG 10 8 17 8 24 0 100 5 10
    240604235445_sswp GTCCCCGTGTCTTAGCTAGTTCTCCATTGTCG(+ACTCAC)GTACCACATCCACTCGTCGTGACTAACCGCT NGG 10 8 17 8 24 0 100 5 10
    240604235444_5z47 TGGGGAGCGGTTAGTCACGACGAGTGGATGTG(+ACTCAC)GTACCGACAATGGAGAACTAGCTAAGACACG NGG 10 8 17 8 24 0 100 5 10
    240604235442_abbx AGAGCTGGGGAGAACCCATTCTTCCTTGGAAG(+ACTCAC)GATCCGCTGAAGGTCAGCGTATAGGTGATGT NGG 10 8 17 8 24 0 100 5 10
    240604235441_svp6 ACTGTACATCACCTATACGCTGACCTTCAGCG(+ACTCAC)GATCCTTCCAAGGAAGAATGGGTTCTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240604235440_sp1w CCTGGCTAAGCATGTTTCAGGTCCTGTAAGTG(+ACTCAC)GTACCCAAGCAGGCGCGTCTTGTTTCTGGTC NGG 10 8 17 8 24 0 100 5 10
    240604235438_k0d6 GTCATGACCAGAAACAAGACGCGCCTGCTTGG(+ACTCAC)GTACCACTTACAGGACCTGAAACATGCTTAG NGG 10 8 17 8 24 0 100 5 10
    240604235437_m01k TAAAGTTAACAGTACAGCACAGCCCACATGTG(+ACTCAC)GATCCATACCAAATCTATCTGATAAATAGTT NGG 10 8 17 8 24 0 100 5 10
    240604235436_42ty GTTTCAACTATTTATCAGATAGATTTGGTATG(+ACTCAC)GATCCACATGTGGGCTGTGCTGTACTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240604235434_3s3t GGAGAGCCCTGCTGCTTTCTGATGTTTGGAAG(+ACTCAC)GTACCCAGCATCCTGATTCCCTCCAGTTCAT NGG 10 8 17 8 24 0 100 5 10