Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604235504_nvx7 GGTGTCCTTGATGCTGGCTGCTCACATGCATG(+ACTCAC)GATCCTCTCCCTGTTCCTGGGCGCTTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240604235503_9whn TTGATATAAAGCACAAACTGGCTTTAAACTCG(+ACTCAC)GATCCTTCGGCCTCAGCCTTTCTAGCACCAA NGG 10 8 17 8 24 0 100 5 10
    240604235501_pta7 TCTCCTTGGTGCTAGAAAGGCTGAGGCCGAAG(+ACTCAC)GATCCGAGTTTAAAGCCAGTTTGTGCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240604235459_uiiq TGTGGGTAGAAAATGGTAACACTAAACAATGG(+ACTCAC)GATCCCAGTACTCAACTTTAAAGATTTTATT NGG 10 8 17 8 24 0 100 5 10
    240604235457_eki5 GAGAAAATAAAATCTTTAAAGTTGAGTACTGG(+ACTCAC)GATCCCATTGTTTAGTGTTACCATTTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240604235455_1uvy ACACCCCTGAAACTGACAAGTGAGGGGCCAGG(+ACTCAC)GATCCCCCACTGACTGGATTGTGTCTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240604235453_u3bx TGATGTGCCCAGACACAATCCAGTCAGTGGGG(+ACTCAC)GATCCCTGGCCCCTCACTTGTCAGTTTCAGG NGG 10 8 17 8 24 0 100 5 10
    240604235451_mr7n AGGAAAAACCTCGCCTTTGACCTGAGAATGGG(+ACTCAC)GTACCGCCCCTCGGGAACACAGTGAGCTCAC NGG 10 8 17 8 24 0 100 5 10
    240604235449_hcln CCCCAGTGAGCTCACTGTGTTCCCGAGGGGCG(+ACTCAC)GTACCCCATTCTCAGGTCAAAGGCGAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240604235448_9v5b AATACAGTGAGAAGTTAATTTATACTCAAGTG(+ACTCAC)GTACCACTTAAAGGCATGTGTTCTTTTAGTA NGG 10 8 17 8 24 0 100 5 10