Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000015_ttir ACGTGGTGAGCATGTGTGAAGACCTAAGTTTG(+ACTCAC)GATCCCCAGAACCCCTATGAAAGCTGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605000014_wc0r TGGACAGCCATGTTGCTATTTGGCATGAAGAG(+ACTCAC)GATCCCACTCAAAGATCCAAGGGAGGAATCT NGG 10 8 17 8 24 0 100 5 10
    240605000012_kvni TCCCAAGATTCCTCCCTTGGATCTTTGAGTGG(+ACTCAC)GATCCTCTTCATGCCAAATAGCAACATGGCT NGG 10 8 17 8 24 0 100 5 10
    240605000011_qfe4 CAGATAGCCTTTGATGCATATAAAGGACAGCG(+ACTCAC)GATCCGCTGCACTCCAGGTGCTGCTCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605000010_gew2 TAGGAGGCTTGAGCAGCACCTGGAGTGCAGCG(+ACTCAC)GATCCGCTGTCCTTTATATGCATCAAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605000008_f9cp GAAGAAGTTGATTTCAAACAAAAATTAAAAGG(+ACTCAC)GTACCCTGACTACTGGGGTGTGTGTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605000006_updn CACACACACACACACACACCCCAGTAGTCAGG(+ACTCAC)GTACCCTTTTAATTTTTGTTTGAAATCAACT NGG 10 8 17 8 24 0 100 5 10
    240605000004_y94k GGCCAGATCGACCAGAAATGGCATCTGCCATG(+ACTCAC)GTACCTGCTGTTTCTAGTAGCAGGCCATGAT NGG 10 8 17 8 24 0 100 5 10
    240605000003_1ayq AAGACATCATGGCCTGCTACTAGAAACAGCAG(+ACTCAC)GTACCATGGCAGATGCCATTTCTGGTCGATC NGG 10 8 17 8 24 0 100 5 10
    240605000001_v9bo GCTCGTACATTGGTCCTTGCCTTCTGGAGGAG(+ACTCAC)GTACCCAGGGCGGTCCTGCTTCCTCTCTTCA NGG 10 8 17 8 24 0 100 5 10