Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605000440_bttd CTGTGTTTGGTAGAGTAACTCAGGTGAAGCTG(+ACTCAC)GATCCCACTGCTATGACAAGCCATCCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605000439_b4d3 GACAGGAGCGCAGAGGTGAGAGAAAACAACAG(+ACTCAC)GATCCTCCCAAGAAGTCCTTTCCTTAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605000437_hice CACAAAGGCCTAAGGAAAGGACTTCTTGGGAG(+ACTCAC)GATCCTGTTGTTTTCTCTCACCTCTGCGCTC NGG 10 8 17 8 24 0 100 5 10
    240605000435_2u09 GAGAATCCTGTTAAAATTGTTGGCTGTTACTG(+ACTCAC)GTACCCTATGTTAATCAGCCACCAGGTGTCA NGG 10 8 17 8 24 0 100 5 10
    240605000433_l3s2 TGAAATGACACCTGGTGGCTGATTAACATAGG(+ACTCAC)GTACCAGTAACAGCCAACAATTTTAACAGGA NGG 10 8 17 8 24 0 100 5 10
    240605000432_murg TGATACAGTCAGCACCAGCAGACCTGCTTCAG(+ACTCAC)GATCCAAGAAAGCTGGCTTTGTAGGCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605000431_35p5 ACTGAAGACAGCCTACAAAGCCAGCTTTCTTG(+ACTCAC)GATCCTGAAGCAGGTCTGCTGGTGCTGACTG NGG 10 8 17 8 24 0 100 5 10
    240605000430_k0gs CAGAACGAACCTTCTCTGGACTCAGATTTACG(+ACTCAC)GATCCCTGTCCATACCCGCTTCAGCTAACTA NGG 10 8 17 8 24 0 100 5 10
    240605000428_q7n5 AAGGCTAGTTAGCTGAAGCGGGTATGGACAGG(+ACTCAC)GATCCGTAAATCTGAGTCCAGAGAAGGTTCG NGG 10 8 17 8 24 0 100 5 10
    240605000426_rlv3 GTTAGGGTTAACGACACTGTTGTACTGTCATG(+ACTCAC)GTACCCCCTGTATAAAGGATCAGTTTGACTC NGG 10 8 17 8 24 0 100 5 10