Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001313_cb9t CACCAGGACAGCTGAGCCTACACCCACATGAG(+ACTCAC)GATCCTGTGTGCTGATTGGGACTCCAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605001311_6da9 TGCAGTTCTGTACATTGACCTGGGACACACCG(+ACTCAC)GTACCAAATACAGGGCTGAGAGTGATTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605001310_qyre CTCTTGCCAAATCACTCTCAGCCCTGTATTTG(+ACTCAC)GTACCGGTGTGTCCCAGGTCAATGTACAGAA NGG 10 8 17 8 24 0 100 5 10
    240605001309_rs5j TAACTAGGAACAGACATCATTGTACACAATAG(+ACTCAC)GTACCAAGAGCAAAAAAAAAAAAAATAGTAG NGG 10 8 17 8 24 0 100 5 10
    240605001307_9agr ATGTGCTACTATTTTTTTTTTTTTTGCTCTTG(+ACTCAC)GTACCTATTGTGTACAATGATGTCTGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605001306_bgok CCCTGTCAATTCTTTATGTCAGCACAGAACTG(+ACTCAC)GATCCTACCAGTTCAGAATAAAGAAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240605001305_ig9j AATTCCAAAGTTTCTTTATTCTGAACTGGTAG(+ACTCAC)GATCCAGTTCTGTGCTGACATAAAGAATTGA NGG 10 8 17 8 24 0 100 5 10
    240605001303_omc3 TAGATCAGGAGGGCTGAATGGATTGGAAGGTG(+ACTCAC)GATCCTATGTGGAGGATATGGGTGACTAGGA NGG 10 8 17 8 24 0 100 5 10
    240605001302_kzke CTTGCTCCTAGTCACCCATATCCTCCACATAG(+ACTCAC)GATCCACCTTCCAATCCATTCAGCCCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605001301_yoi2 TCGTGCTATATGGGTTATTTTGGTCTTGTAAG(+ACTCAC)GATCCGTTCCTTTAAAATAATGGTCTTAGAA NGG 10 8 17 8 24 0 100 5 10