Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605001622_b4tn GCGAGCCCTCCCCCCCCCCCCAGATGTCTCAG(+ACTCAC)GATCCCCCCTTGGGGCCAGCAGAATGGGCCC NGG 10 8 17 8 24 0 100 5 10
    240605001620_2kdw GTTCCTGTAATAGAAGTTATTAGTGCAGGCGG(+ACTCAC)GATCCGGCACAGCCAGCTCCTGGCATCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605001619_x6m2 TGAGGGCAGGATGCCAGGAGCTGGCTGTGCCG(+ACTCAC)GATCCCGCCTGCACTAATAACTTCTATTACA NGG 10 8 17 8 24 0 100 5 10
    240605001617_lwt2 AGATACCTCCGTTTTCTTTGTGACCGGCGTTG(+ACTCAC)GATCCTGGATAGTTATGGCTGGCTGCAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605001615_berk CGCAGTGACTGCAGCCAGCCATAACTATCCAG(+ACTCAC)GATCCAACGCCGGTCACAAAGAAAACGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605001613_rthm CACCAGCCCAGCTCCTGTCCACCGCTGCTCGG(+ACTCAC)GTACCCAGGGCTACCCCAGACCCTGCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605001610_p8mn GAGCCTCTCTGCAGGGTCTGGGGTAGCCCTGG(+ACTCAC)GTACCCGAGCAGCGGTGGACAGGAGCTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605001609_4hhp GGCCTCAGTGCTCAGCCTGCTCTTTAATAAAG(+ACTCAC)GTACCTCTTTTCTGAGCCATGTCGGAATGAG NGG 10 8 17 8 24 0 100 5 10
    240605001608_ic0u CTCCACTCATTCCGACATGGCTCAGAAAAGAG(+ACTCAC)GTACCTTTATTAAAGAGCAGGCTGAGCACTG NGG 10 8 17 8 24 0 100 5 10
    240605001606_v1bw CCTTACCCTGTACTAGGCCCATCAGTCACAGG(+ACTCAC)GTACCTGGGGCCACTATGCACCATGGTCAGT NGG 10 8 17 8 24 0 100 5 10