Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161010_bt7m ACGGATCTGAGGAGATGGAGGTGGATGTTGCG(+AGGACA)GATCCCAGCTGGGGGCTACTTGTCGATGAGC NGG 10 8 17 8 24 0 100 5 10
    240613161009_h3h8 TCCTCCTTCCCCTGGCTAGCACTCTGGTCAGG(+AGGACA)GTACCCCTTTATTCTGCTGCAGAGATCTTAA NGG 10 8 17 8 24 0 100 5 10
    240613161006_dul6 TGTGTTTAAGATCTCTGCAGCAGAATAAAGGG(+AGGACA)GTACCCTGACCAGAGTGCTAGCCAGGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613161005_0sj9 CCATTCCCCCATTCGACATGGGATTTCACCAG(+AGGACA)GATCCACCTATATCCCCCTTTTTTTGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613161003_p9s9 AAATATCTTTCAAAAAAAGGGGGATATAGGTG(+AGGACA)GATCCTGGTGAAATCCCATGTCGAATGGGGG NGG 10 8 17 8 24 0 100 5 10
    240613161001_yao1 GTGTATGTTAGTGGCAGGAAATTCAGAGTTGG(+AGGACA)GATCCCTGATAGGAGGCAGATCCTGGACTGG NGG 10 8 17 8 24 0 100 5 10
    240613161000_b0fm CGCGCCCAGTCCAGGATCTGCCTCCTATCAGG(+AGGACA)GATCCCAACTCTGAATTTCCTGCCACTAACA NGG 10 8 17 8 24 0 100 5 10
    240613160958_2ay0 GTGTATGTTAGTGGCAGGAAATTCAGAGTTGG(+AGGACA)GATCCCTGATAGGAGGCAGATCCTGGACTGG NGG 10 8 17 8 24 0 100 5 10
    240613160956_wkzw CGCGCCCAGTCCAGGATCTGCCTCCTATCAGG(+AGGACA)GATCCCAACTCTGAATTTCCTGCCACTAACA NGG 10 8 17 8 24 0 100 5 10
    240613160955_pi42 TGCCAGCTCAGGGTCCCCTCCACTGGTTCTTG(+AGGACA)GTACCTACCACCCTCCTTTAAAGACCCAGAG NGG 10 8 17 8 24 0 100 5 10