Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613161212_zp5w GTCTACATCCTGGCTGGCTGGATGCCTACCTG(+AGGACA)GTACCCACTTGCCATGCAGAGGCCTGTCCGG NGG 10 8 17 8 24 0 100 5 10
    240613161211_ybkr ACCTCCCGGACAGGCCTCTGCATGGCAAGTGG(+AGGACA)GTACCAGGTAGGCATCCAGCCAGCCAGGATG NGG 10 8 17 8 24 0 100 5 10
    240613161210_c4e0 GAGCAGCTTCTAGCCTGGGTCACGAAGAGCAG(+AGGACA)GTACCCCTAGGAAGGCAGGAGAGTGTCTAGG NGG 10 8 17 8 24 0 100 5 10
    240613161208_9cm6 GAACCCCTAGACACTCTCCTGCCTTCCTAGGG(+AGGACA)GTACCTGCTCTTCGTGACCCAGGCTAGAAGC NGG 10 8 17 8 24 0 100 5 10
    240613161205_p7wp CTAACAACTGTCTGCAACTCCAGTCCCAAGGG(+AGGACA)GATCCAAAGGTCTCTTCTGGCCTCTGCACAT NGG 10 8 17 8 24 0 100 5 10
    240613161203_cyfl ACCACATGTGCAGAGGCCAGAAGAGACCTTTG(+AGGACA)GATCCCCTTGGGACTGGAGTTGCAGACAGTT NGG 10 8 17 8 24 0 100 5 10
    240613161202_c4py ATTTTTGCAAATAGATAAATTTAACAATCTTG(+AGGACA)GTACCCCACCTCAAAAGTGCAGTCACACAAA NGG 10 8 17 8 24 0 100 5 10
    240613161200_ech7 GCTGGTTTGTGTGACTGCACTTTTGAGGTGGG(+AGGACA)GTACCAAGATTGTTAAATTTATCTATTTGCA NGG 10 8 17 8 24 0 100 5 10
    240613161159_sbnc TAGGCTTCCTTCTCCTTGGAGGCCTGAGTTAG(+AGGACA)GTACCATGTCCACAACACTACAACTCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240613161157_hkyl AGAGTCAAGAGAGTTGTAGTGTTGTGGACATG(+AGGACA)GTACCTAACTCAGGCCTCCAAGGAGAAGGAA NGG 10 8 17 8 24 0 100 5 10