Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160924_r84n CAACCCCAAACTTTTAAATCTCTGGGCCAGTG(+AGGACA)GATCCAAGAGGCCCTCAGCAAACCGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613160922_kc7v AGTATATCTCTGCCCCAGCTCCAGTCTTGCTG(+AGGACA)GATCCTCCTCCAGCTCCAACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240613160921_g9hc GTGTGTGTGTGTGTGTGTTGGAGCTGGAGGAG(+AGGACA)GATCCAGCAAGACTGGAGCTGGGGCAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240613160919_rdu2 CGACTCGGCGGTGAACGAACCTCAGGGTGCGG(+AGGACA)GATCCTACACACACTCCCTTTCCCCACCCCC NGG 10 8 17 8 24 0 100 5 10
    240613160918_t8jy GGGTGGGGGGTGGGGAAAGGGAGTGTGTGTAG(+AGGACA)GATCCCGCACCCTGAGGTTCGTTCACCGCCG NGG 10 8 17 8 24 0 100 5 10
    240613160917_w8as CACTGCCTTCTCCTTCCTGTCCCCCCTGGAAG(+AGGACA)GTACCCAGTTCTGTCTGAGAGGACAAGCGGG NGG 10 8 17 8 24 0 100 5 10
    240613160915_2h0g CCGGGCCCGCTTGTCCTCTCAGACAGAACTGG(+AGGACA)GTACCTTCCAGGGGGGACAGGAAGGAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613160914_yenv CGGAGGCGGGGAGGCCGCTCCTCTTTGGGCAG(+AGGACA)GATCCTACTATGGACACAGAACAGACAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613160912_0jo3 GGCAGCTGCTGTCTGTTCTGTGTCCATAGTAG(+AGGACA)GATCCTGCCCAAAGAGGAGCGGCCTCCCCGC NGG 10 8 17 8 24 0 100 5 10
    240613160911_1knq ATTAGAAAGGGTGGCAGGTCAAGTCGAGGAAG(+AGGACA)GTACCATCAGCCCAAGCAAAAAGCCCGGGTT NGG 10 8 17 8 24 0 100 5 10