Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160953_1804 GGTGCCTCTGGGTCTTTAAAGGAGGGTGGTAG(+AGGACA)GTACCAAGAACCAGTGGAGGGGACCCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240613160952_kpju TGTCTGTTCCTGCTTTTCTGTCCTGGGCTGGG(+AGGACA)GATCCAGGCTCAGCACTTTCTCCCCGTCCCC NGG 10 8 17 8 24 0 100 5 10
    240613160950_91se ATAGTGGGGACGGGGAGAAAGTGCTGAGCCTG(+AGGACA)GATCCCCAGCCCAGGACAGAAAAGCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240613160948_6vpj TTAAGAAATTAATATAGAAGGAGATGAAAGTG(+AGGACA)GATCCTGAAAGGGTGACAGGAGGAATGGAAA NGG 10 8 17 8 24 0 100 5 10
    240613160947_pbmk TAACATTTCCATTCCTCCTGTCACCCTTTCAG(+AGGACA)GATCCACTTTCATCTCCTTCTATATTAATTT NGG 10 8 17 8 24 0 100 5 10
    240613160946_7wwy AACCTGTTCCTGGAGGTGGAACTCTTCATCAG(+AGGACA)GATCCCCAAAGGCGTGCTGGAATTAGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240613160944_apdj GTTCAGTGGACTAATTCCAGCACGCCTTTGGG(+AGGACA)GATCCTGATGAAGAGTTCCACCTCCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240613160943_m29e TTCTATAACCAATACCTTGACCCTCTACAGTG(+AGGACA)GATCCAGAAATGCTGAGAACCCACAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613160941_20ov GGTGGTCAGCCTGTGGGTTCTCAGCATTTCTG(+AGGACA)GATCCACTGTAGAGGGTCAAGGTATTGGTTA NGG 10 8 17 8 24 0 100 5 10
    240613160939_4v4t AATAGGCCAGACCATTCTACACTTTTCCACTG(+AGGACA)GATCCTTTCCTTATTTTTTTTGGATCATAAT NGG 10 8 17 8 24 0 100 5 10