Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160853_32d5 AGGGGACGGACCAGAGTGCCTATAGAGAGGAG(+AGGACA)GTACCAGTAGACAGCTCCTTCTGTCCCCGGA NGG 10 8 17 8 24 0 100 5 10
    240613160851_gknr TCCTAAAATATTTTTAAAAGGGAAGATTAATG(+AGGACA)GTACCAAGAACTAAGATCTAGGTAGATGAGA NGG 10 8 17 8 24 0 100 5 10
    240613160850_4dvi AACCATCTCATCTACCTAGATCTTAGTTCTTG(+AGGACA)GTACCATTAATCTTCCCTTTTAAAAATATTT NGG 10 8 17 8 24 0 100 5 10
    240613160849_qmc7 GGGGATCCAACACCTTCTTCTGGCCTCTGTAG(+AGGACA)GTACCAGGCATGCAAAAAACAAAACAAAACA NGG 10 8 17 8 24 0 100 5 10
    240613160847_bapf TGTTTTGTTTTGTTTTGTTTTTTGCATGCCTG(+AGGACA)GTACCTACAGAGGCCAGAAGAAGGTGTTGGA NGG 10 8 17 8 24 0 100 5 10
    240613160846_eeci TTGCCACTCTTGCAACTCCTATCACCCTCATG(+AGGACA)GTACCTTATAACCATTTATAATTCCAGTTCG NGG 10 8 17 8 24 0 100 5 10
    240613160845_kskx TTCCTCGAACTGGAATTATAAATGGTTATAAG(+AGGACA)GTACCATGAGGGTGATAGGAGTTGCAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240613160843_l75l AAGTGCCCCTAGCTGCTTCTCTCTGGTCCAAG(+AGGACA)GTACCTGATTCTAGTAAGGAGAGCACTGAGC NGG 10 8 17 8 24 0 100 5 10
    240613160842_cm3f ATTGTGCTCAGTGCTCTCCTTACTAGAATCAG(+AGGACA)GTACCTTGGACCAGAGAGAAGCAGCTAGGGG NGG 10 8 17 8 24 0 100 5 10
    240613160839_hf7x ATTCACAACCATCTGTAACCTGAGTTTCAGGG(+AGGACA)GATCCAACACCTTCTTCTGGCCTCTGTAGGT NGG 10 8 17 8 24 0 100 5 10