Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160822_2grg CCACCTGGAGGCTTAGGGCACCATCTTTGAGG(+AGGACA)GTACCTTATTCTTCCTTTTGCTTGGCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240613160820_ltoy TCCGGTCAGGCCGCTCAGGGACAGATGGATGG(+AGGACA)GTACCTGGTGCTGTCTGCAGAACTCAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240613160819_r659 GACTGGAGACTGAGTTCTGCAGACAGCACCAG(+AGGACA)GTACCCATCCATCTGTCCCTGAGCGGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613160816_ld55 GAATTGTTGTGACTTCCACGGATGTACCATGG(+AGGACA)GATCCATGTGCGTTAATAGAAAGGATTATGT NGG 10 8 17 8 24 0 100 5 10
    240613160815_vzpc ATTTTACATAATCCTTTCTATTAACGCACATG(+AGGACA)GATCCCATGGTACATCCGTGGAAGTCACAAC NGG 10 8 17 8 24 0 100 5 10
    240613160814_5uha CTGAGGCCCCAGGGGACAGGCTCTATGTGTTG(+AGGACA)GTACCCGTGAGGTGACCACCATCCAGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240613160812_hgyl TAGGAAGCCCCTGGATGGTGGTCACCTCACGG(+AGGACA)GTACCAACACATAGAGCCTGTCCCCTGGGGC NGG 10 8 17 8 24 0 100 5 10
    240613160809_bt7z CCGCTGAATCTTCCCCAATCCCCCAGACCCGG(+AGGACA)GATCCCCCACCATGTCCATCCCATAACATAA NGG 10 8 17 8 24 0 100 5 10
    240613160805_mh7j AACTGTTATGTTATGGGATGGACATGGTGGGG(+AGGACA)GATCCCGGGTCTGGGGGATTGGGGAAGATTC NGG 10 8 17 8 24 0 100 5 10
    240613160803_y2jd CAAACTTTGTGGCTTAGAAGCCTGAAGAAGGG(+AGGACA)GATCCCTTCCCTCCGAGAGCGCTTAACCCTG NGG 10 8 17 8 24 0 100 5 10