Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233557_hk3b AGGGCATCCTGCCGCGCCGAGGGAGCATCTGG(+ACTCAC)GATCCACACGTCTCCTGTCCCTGCAGACCTG NGG 10 8 17 8 24 0 100 5 10
    240604233556_qlt9 GTGCTCAGGTCTGCAGGGACAGGAGACGTGTG(+ACTCAC)GATCCCAGATGCTCCCTCGGCGCGGCAGGAT NGG 10 8 17 8 24 0 100 5 10
    240604233553_z86z GCACCTGCCTAGGGGAGGCAGCTTTCTGAGGG(+ACTCAC)GATCCAGAGACAATGGCCAGTGGCAAATGTT NGG 10 8 17 8 24 0 100 5 10
    240604233552_yrzl TCACAAACATTTGCCACTGGCCATTGTCTCTG(+ACTCAC)GATCCCCTCAGAAAGCTGCCTCCCCTAGGCA NGG 10 8 17 8 24 0 100 5 10
    240604233550_jngh AGAACACAGCCTGTGCCTGAATTAATGGCTGG(+ACTCAC)GATCCATGGATGGAACACTTGGGTATGCACA NGG 10 8 17 8 24 0 100 5 10
    240604233548_mmza GGCTTTGTGCATACCCAAGTGTTCCATCCATG(+ACTCAC)GATCCCAGCCATTAATTCAGGCACAGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240604233545_znfy TGGGTTTGTGGCCACTCCAGGGGTTTACCCTG(+ACTCAC)GATCCTCTGCCTCCGAGGGGCTTGTCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240604233544_25ri GAAAGATTAGGACAAGCCCCTCGGAGGCAGAG(+ACTCAC)GATCCAGGGTAAACCCCTGGAGTGGCCACAA NGG 10 8 17 8 24 0 100 5 10
    240604233543_q12t TCACTTGTGGGTAGAGGGCAGCTCTCTCGGTG(+ACTCAC)GATCCCCACAGCCAATCAAGCTGTAATCGCA NGG 10 8 17 8 24 0 100 5 10
    240604233541_662x TCTCCTGCGATTACAGCTTGATTGGCTGTGGG(+ACTCAC)GATCCACCGAGAGAGCTGCCCTCTACCCACA NGG 10 8 17 8 24 0 100 5 10