Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233649_6s59 AACAAACAAACAAACAAACATATTATTAGAGG(+ACTCAC)GATCCTACCAAGGCCTTCTGGTCAACTCTAC NGG 10 8 17 8 24 0 100 5 10
    240604233647_vjbu CAAGAGTAGAGTTGACCAGAAGGCCTTGGTAG(+ACTCAC)GATCCCTCTAATAATATGTTTGTTTGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240604233645_kq5j TACATATGCAGCTAGAGACACAAACTCAGGGG(+ACTCAC)GTACCTGTTAGTTCATATTGTTGTTCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240604233644_q6lg CCTATAGGAGGAACAACAATATGAACTAACAG(+ACTCAC)GTACCCCCTGAGTTTGTGTCTCTAGCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240604233643_cc8d TGTGTCTGTCTTTGGTGGTTGATTATGGTATG(+ACTCAC)GATCCCTGGATGGGACAGTCTCTGCATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604233640_qfmc GGAAAGACCATGCAGAGACTGTCCCATCCAGG(+ACTCAC)GATCCATACCATAATCAACCACCAAAGACAG NGG 10 8 17 8 24 0 100 5 10
    240604233638_0rbt TTTTGGCCCTGTTGATCTCGACTGAATGCCAG(+ACTCAC)GTACCAGTCTTCTCTACCTTCCCTGCCAAAG NGG 10 8 17 8 24 0 100 5 10
    240604233637_1w90 TGAGCCTTTGGCAGGGAAGGTAGAGAAGACTG(+ACTCAC)GTACCTGGCATTCAGTCGAGATCAACAGGGC NGG 10 8 17 8 24 0 100 5 10
    240604233634_otfi AAAAAGCTGCTTCTAGTGTGTGAGGTTTCCGG(+ACTCAC)GTACCTTTTAGTTCCTCATAAAGTTGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240604233633_2vee TCAGAGTCTGCAACTTTATGAGGAACTAAAAG(+ACTCAC)GTACCCGGAAACCTCACACACTAGAAGCAGC NGG 10 8 17 8 24 0 100 5 10