Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233705_f58w TTGTTCCTGCATGAGATGGCTTTGGGTGTCTG(+ACTCAC)GTACCACTCCTTCCCTTCCCCCTACCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240604233704_tnng CAATCGGCCAGGTAGGGGGAAGGGAAGGAGTG(+ACTCAC)GTACCAGACACCCAAAGCCATCTCATGCAGG NGG 10 8 17 8 24 0 100 5 10
    240604233703_9x44 GTAGTAGTAGTAGTAGTTCCATGTAGGGGCAG(+ACTCAC)GATCCTTTGGAAAAGTACCGTATGTTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240604233701_nl9r GGGGTCTAGGAACATACGGTACTTTTCCAAAG(+ACTCAC)GATCCTGCCCCTACATGGAACTACTACTACT NGG 10 8 17 8 24 0 100 5 10
    240604233659_xd4t GGGGTCAAGTTGGTCACAGGTAGGAATCTGGG(+ACTCAC)GTACCATCAGGGTCTAGTGAATGAAGGATTC NGG 10 8 17 8 24 0 100 5 10
    240604233657_uan2 AGGCTGAATCCTTCATTCACTAGACCCTGATG(+ACTCAC)GTACCCCAGATTCCTACCTGTGACCAACTTG NGG 10 8 17 8 24 0 100 5 10
    240604233656_5qld GTTTGAAAGGTGAACTGTAAGACTCTTCACTG(+ACTCAC)GATCCTTAATGTTTCAGTGGTCCAGTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240604233655_453o TTTGTTGAAGACTGGACCACTGAAACATTAAG(+ACTCAC)GATCCAGTGAAGAGTCTTACAGTTCACCTTT NGG 10 8 17 8 24 0 100 5 10
    240604233653_g5x8 GGAAGGGCTATCCAGAGACTGCTCCACTTGGG(+ACTCAC)GATCCATCCCATATCCAGTCACCAAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240604233651_7nrd AGTGTCTGGATTTGGTGACTGGATATGGGATG(+ACTCAC)GATCCCCAAGTGGAGCAGTCTCTGGATAGCC NGG 10 8 17 8 24 0 100 5 10