Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233720_nkha GTGAAGATGGATACCTAGTCCTTCCCCGCTTG(+ACTCAC)GTACCCACCAAGAGTAACAGGGAGATATGTG NGG 10 8 17 8 24 0 100 5 10
    240604233718_rldx CCGCACACATATCTCCCTGTTACTCTTGGTGG(+ACTCAC)GTACCAAGCGGGGAAGGACTAGGTATCCATC NGG 10 8 17 8 24 0 100 5 10
    240604233717_drcl CTTGGGGGCCTATCCCTTAGAACTCTGGACTG(+ACTCAC)GATCCTGAGCCATTTTTCGAAATGAAAAGTG NGG 10 8 17 8 24 0 100 5 10
    240604233715_0hp5 CTTCCCACTTTTCATTTCGAAAAATGGCTCAG(+ACTCAC)GATCCAGTCCAGAGTTCTAAGGGATAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240604233714_x3hy GGTGGGTGGGCAGGAGCTGGAGCCATTCTTAG(+ACTCAC)GTACCAACCCTCCCAGACTCCTAGCTAGACA NGG 10 8 17 8 24 0 100 5 10
    240604233712_vli7 TGCTGTGTCTAGCTAGGAGTCTGGGAGGGTTG(+ACTCAC)GTACCTAAGAATGGCTCCAGCTCCTGCCCAC NGG 10 8 17 8 24 0 100 5 10
    240604233711_3cz3 TTGTTCCTGCATGAGATGGCTTTGGGTGTCTG(+ACTCAC)GTACCACTCCTTCCCTTCCCCCTACCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240604233710_c6bg CAATCGGCCAGGTAGGGGGAAGGGAAGGAGTG(+ACTCAC)GTACCAGACACCCAAAGCCATCTCATGCAGG NGG 10 8 17 8 24 0 100 5 10
    240604233708_0aez GTAGTAGTAGTAGTAGTTCCATGTAGGGGCAG(+ACTCAC)GATCCTTTGGAAAAGTACCGTATGTTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240604233706_8ie4 GGGGTCTAGGAACATACGGTACTTTTCCAAAG(+ACTCAC)GATCCTGCCCCTACATGGAACTACTACTACT NGG 10 8 17 8 24 0 100 5 10