Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233825_74bk TTTGCAGCTGTCAGTTGTTCTTTAAACTCTAG(+ACTCAC)GATCCATCTTTGGACCAAAAGTCTGGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240604233824_ij1d TAGGCCCAGACCAGACTTTTGGTCCAAAGATG(+ACTCAC)GATCCTAGAGTTTAAAGAACAACTGACAGCT NGG 10 8 17 8 24 0 100 5 10
    240604233822_66xe GGCTGTACTCTACACACTTCCTAGTCACGCAG(+ACTCAC)GATCCCTGTAGCCAAAACAGTATATGTTCCA NGG 10 8 17 8 24 0 100 5 10
    240604233821_6fyn GCAGCTGGAACATATACTGTTTTGGCTACAGG(+ACTCAC)GATCCTGCGTGACTAGGAAGTGTGTAGAGTA NGG 10 8 17 8 24 0 100 5 10
    240604233820_eg3i GACGCCATTGGAGTAGATCACCGCAGGTGACG(+ACTCAC)GTACCAGCCTGAAGCCCCACCCAAAAGGCGC NGG 10 8 17 8 24 0 100 5 10
    240604233818_792o GGTAGGCGCCTTTTGGGTGGGGCTTCAGGCTG(+ACTCAC)GTACCGTCACCTGCGGTGATCTACTCCAATG NGG 10 8 17 8 24 0 100 5 10
    240604233816_qzd2 GTAATTTTCATAATTGCTAAATGGAATTCATG(+ACTCAC)GATCCCATACAATGCCGCCCAACACTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240604233815_thph TCCAAGCTCAAGTGTTGGGCGGCATTGTATGG(+ACTCAC)GATCCATGAATTCCATTTAGCAATTATGAAA NGG 10 8 17 8 24 0 100 5 10
    240604233813_ojsb TGGATGAGGAGTGTGTCGCTATAACCACTCTG(+ACTCAC)GTACCCAAGAGGGGCTGGGCACCTACTCGGG NGG 10 8 17 8 24 0 100 5 10
    240604233811_a381 AGTGTCCCGAGTAGGTGCCCAGCCCCTCTTGG(+ACTCAC)GTACCAGAGTGGTTATAGCGACACACTCCTC NGG 10 8 17 8 24 0 100 5 10