Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233842_dnf5 TGTGAGGGAAGTCGCTTCCCGCGAGATGCTGG(+ACTCAC)GATCCAGCCTGGTTGTCTTTCCGTAAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604233840_fsjp CACAGCCACTTTACGGAAAGACAACCAGGCTG(+ACTCAC)GATCCCAGCATCTCGCGGGAAGCGACTTCCC NGG 10 8 17 8 24 0 100 5 10
    240604233839_x028 TTTTCCCATCCCAGCTTCCTTCTGGTAAACTG(+ACTCAC)GTACCCTCAGGATAAAGAAGTATATGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240604233837_frdn AGGCAGGCACCATATACTTCTTTATCCTGAGG(+ACTCAC)GTACCAGTTTACCAGAAGGAAGCTGGGATGG NGG 10 8 17 8 24 0 100 5 10
    240604233836_d7y1 AATAATTTTTTTACAAAAAAAATTCTATCAAG(+ACTCAC)GATCCTTTAAATATCAAGTTTCCCAATGCAC NGG 10 8 17 8 24 0 100 5 10
    240604233835_h2i6 TCTAAGTGCATTGGGAAACTTGATATTTAAAG(+ACTCAC)GATCCTTGATAGAATTTTTTTTGTAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240604233832_cnln AAAAGAGGCAAACCCACAAGTCCCTGCCTTGG(+ACTCAC)GATCCTAGTTCCTAGCAGGGGTCAGCTTATC NGG 10 8 17 8 24 0 100 5 10
    240604233831_xqo0 CCTTGGATAAGCTGACCCCTGCTAGGAACTAG(+ACTCAC)GATCCCAAGGCAGGGACTTGTGGGTTTGCCT NGG 10 8 17 8 24 0 100 5 10
    240604233828_tetw CACTGTAGATGCTACAAACATGTCTACCCAGG(+ACTCAC)GTACCACTGAGCTACGTCTTCCTCATGGGGC NGG 10 8 17 8 24 0 100 5 10
    240604233827_w1zq CGTCTGCCCCATGAGGAAGACGTAGCTCAGTG(+ACTCAC)GTACCCTGGGTAGACATGTTTGTAGCATCTA NGG 10 8 17 8 24 0 100 5 10