Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233917_2h4f GTGGAATGTGGCCCTAGGATGTACTGGTTTAG(+ACTCAC)GATCCTAGGAAGGTAATCAATCATCCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240604233916_6wix GACACTCTGAGGATGATTGATTACCTTCCTAG(+ACTCAC)GATCCTAAACCAGTACATCCTAGGGCCACAT NGG 10 8 17 8 24 0 100 5 10
    240604233914_y6ni TGGACATTCGGGCACAGATAAGATGCTTGCAG(+ACTCAC)GATCCTGTTAGGGTTTTTCTTGCTGGTTCCG NGG 10 8 17 8 24 0 100 5 10
    240604233913_jkz1 ATCAACGGAACCAGCAAGAAAAACCCTAACAG(+ACTCAC)GATCCTGCAAGCATCTTATCTGTGCCCGAAT NGG 10 8 17 8 24 0 100 5 10
    240604233912_cwcs TCTCTCTCTTCAGACACCAGAAGGTGGCATAG(+ACTCAC)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240604233910_zv1r ACCACATGGTGGCTCACAACCATCTGTAATGG(+ACTCAC)GATCCTATGCCACCTTCTGGTGTCTGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240604233908_aidk GGGTGGGGGCGGGATAGGGGCAGTTAAACAAG(+ACTCAC)GTACCCTGGGGAAGAAATGAAGGTCAGATCC NGG 10 8 17 8 24 0 100 5 10
    240604233905_52ak CCATTGGATCTGACCTTCATTTCTTCCCCAGG(+ACTCAC)GTACCTTGTTTAACTGCCCCTATCCCGCCCC NGG 10 8 17 8 24 0 100 5 10
    240604233903_f7vr TTTCAGGACACCTTGTGTCATGTGTGTTCAGG(+ACTCAC)GATCCTAACTTGGATCTTCATGATTGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240604233902_anj3 TCTGACTTTGCAATCATGAAGATCCAAGTTAG(+ACTCAC)GATCCCTGAACACACATGACACAAGGTGTCC NGG 10 8 17 8 24 0 100 5 10