Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234025_f1j6 TGACAGCTCACAACTGTCTGAACCCTTCACTG(+ACTCAC)GTACCAGGCACCATGGGGTGCACAGAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240604234023_h1lf CATGTATCTCTCTGTGCACCCCATGGTGCCTG(+ACTCAC)GTACCAGTGAAGGGTTCAGACAGTTGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240604234022_twk6 TGGCCAGGCTCAAGCTGGGGTTTTCCAACTCG(+ACTCAC)GATCCAGTCAAGAAAAATCCCTCACAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240604234021_h92u TGGGCACACCTGTGAGGGATTTTTCTTGACTG(+ACTCAC)GATCCGAGTTGGAAAACCCCAGCTTGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240604234019_vo7r GCTAGGCTGGAGGGATCTCCAGGGACAGTGTG(+ACTCAC)GTACCTGGGAAGGGGGCTACTGTGGTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240604234017_wutz GGCTAACAAGACCACAGTAGCCCCCTTCCCAG(+ACTCAC)GTACCACACTGTCCCTGGAGATCCCTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240604234015_fdkt GCCCTAGAAGAAAGCTACGACAGAAAGAGTGG(+ACTCAC)GTACCTCAGGGGTAGATTAGTGACCTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604234013_0cgh TGTCTCTGGGAGGTCACTAATCTACCCCTGAG(+ACTCAC)GTACCCACTCTTTCTGTCGTAGCTTTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240604234012_z3tx GCTTTCCACATTATTCCCCTCACACAAGCCTG(+ACTCAC)GATCCCTCACACTAACTATCTAGAGTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240604234010_1sf5 AACACCTGCCACTCTAGATAGTTAGTGTGAGG(+ACTCAC)GATCCAGGCTTGTGTGAGGGGAATAATGTGG NGG 10 8 17 8 24 0 100 5 10