Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234040_x0pi TGCTGAACCTCACTCTCCCTTCAACCTCCCCG(+ACTCAC)GATCCAAACCAGCTCCAGGAGGAGGGACAGG NGG 10 8 17 8 24 0 100 5 10
    240604234039_cnpi CACCGCCTGTCCCTCCTCCTGGAGCTGGTTTG(+ACTCAC)GATCCGGGGAGGTTGAAGGGAGAGTGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240604234038_ehrz ACAGGTCCGTCCCGGAGCACACAGAGGTGTTG(+ACTCAC)GTACCAAGACTGAAACCTTCACAGATTCTAT NGG 10 8 17 8 24 0 100 5 10
    240604234036_kowk GCCCAATAGAATCTGTGAAGGTTTCAGTCTTG(+ACTCAC)GTACCAACACCTCTGTGTGCTCCGGGACGGA NGG 10 8 17 8 24 0 100 5 10
    240604234035_d6uo GCAGGTCGGGGTTCTCTCAAGTCCCCCAGTCG(+ACTCAC)GATCCGGGACCCCTTTCTGGGCTAGGAAGCG NGG 10 8 17 8 24 0 100 5 10
    240604234033_1f48 GCGGGCGCTTCCTAGCCCAGAAAGGGGTCCCG(+ACTCAC)GATCCGACTGGGGGACTTGAGAGAACCCCGA NGG 10 8 17 8 24 0 100 5 10
    240604234032_sk6w CTGGAGGGATATAAAGGGAACACAGACTTGAG(+ACTCAC)GTACCACAGGCTTTGTCTGAAGAGAGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240604234029_0jlb GTGACAGGGACTCTCTTCAGACAAAGCCTGTG(+ACTCAC)GTACCTCAAGTCTGTGTTCCCTTTATATCCC NGG 10 8 17 8 24 0 100 5 10
    240604234028_d6e7 GGTGACCCAAGCCCAGTGACATCCCTTCAGGG(+ACTCAC)GATCCATGTGAGCCTTCCAGGTACTAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240604234026_5mbl CCGCTGTTTCTAGTACCTGGAAGGCTCACATG(+ACTCAC)GATCCCCTGAAGGGATGTCACTGGGCTTGGG NGG 10 8 17 8 24 0 100 5 10