Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234116_mdzz TGTTCAGTATCCATAGGAAAATAAAACTGCTG(+ACTCAC)GTACCACAGCAGGTTGAAGTACACACCCTCA NGG 10 8 17 8 24 0 100 5 10
    240604234114_uh90 TGGGCTGAGGGTGTGTACTTCAACCTGCTGTG(+ACTCAC)GTACCAGCAGTTTTATTTTCCTATGGATACT NGG 10 8 17 8 24 0 100 5 10
    240604234113_h2dd TGTTCAGTATCCATAGGAAAATAAAACTGCTG(+ACTCAC)GTACCACAGCAGGTTGAAGTACACACCCTCA NGG 10 8 17 8 24 0 100 5 10
    240604234112_i5wj TGGGCTGAGGGTGTGTACTTCAACCTGCTGTG(+ACTCAC)GTACCAGCAGTTTTATTTTCCTATGGATACT NGG 10 8 17 8 24 0 100 5 10
    240604234109_8a0h AGATGGTGCTACAACTGCCCAGTCTATCCTGG(+ACTCAC)GTACCCCACTCAGCCACATCCCTGTCCACAA NGG 10 8 17 8 24 0 100 5 10
    240604234108_aj00 GGGATTTGTGGACAGGGATGTGGCTGAGTGGG(+ACTCAC)GTACCCAGGATAGACTGGGCAGTTGTAGCAC NGG 10 8 17 8 24 0 100 5 10
    240604234106_6t2a GATGATCCGTGAGGATGTGGAGGGGTGGGCTG(+ACTCAC)GATCCAGGCTCAGGGCCGGCATCGTTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240604234104_8jj7 GGGCAGAGCAAACGATGCCGGCCCTGAGCCTG(+ACTCAC)GATCCAGCCCACCCCTCCACATCCTCACGGA NGG 10 8 17 8 24 0 100 5 10
    240604234102_mqih TGTGTGATCATCCCCGGCACCCATTCTACCTG(+ACTCAC)GATCCCACCCGCCAAGGCTGGACCAGATCGA NGG 10 8 17 8 24 0 100 5 10
    240604234100_tl37 TAACCTCGATCTGGTCCAGCCTTGGCGGGTGG(+ACTCAC)GATCCAGGTAGAATGGGTGCCGGGGATGATC NGG 10 8 17 8 24 0 100 5 10