Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234203_6siz TTCTCGGTTAAGGTTGGACAGTCATTTCATTG(+ACTCAC)GTACCAGACCACATTTGCAGCAAGATTTCAC NGG 10 8 17 8 24 0 100 5 10
    240604234202_hlfd ACAAGGTGAAATCTTGCTGCAAATGTGGTCTG(+ACTCAC)GTACCAATGAAATGACTGTCCAACCTTAACC NGG 10 8 17 8 24 0 100 5 10
    240604234200_w0zc AAGAGCCACACCCAACAAGACATGGGGTAGGG(+ACTCAC)GATCCTGTGTCATACTTGAGGCTCTGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240604234158_6y39 GCGCCAAAGCCAGAGCCTCAAGTATGACACAG(+ACTCAC)GATCCCCTACCCCATGTCTTGTTGGGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240604234156_ly97 GCTGTGGGTCCTCCTGCATTTCTTCACCCTAG(+ACTCAC)GATCCTGCTTCCTCAGGTGGAGGAAGGAGCC NGG 10 8 17 8 24 0 100 5 10
    240604234155_3zil CTATGGGCTCCTTCCTCCACCTGAGGAAGCAG(+ACTCAC)GATCCTAGGGTGAAGAAATGCAGGAGGACCC NGG 10 8 17 8 24 0 100 5 10
    240604234154_odph GGACACACCTCAGGATCATTATCCCAGGAATG(+ACTCAC)GTACCACCCACAGTGGGTGGGGCTCTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240604234152_lmg5 TGGACGTGGGAGAGCCCCACCCACTGTGGGTG(+ACTCAC)GTACCATTCCTGGGATAATGATCCTGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240604234150_ipih GGCCCTGCTGGCCTAGCGCTGTTTCTGTGGAG(+ACTCAC)GATCCTCCTGTCTCAGCCTTCTGAGTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240604234149_wrpj CAAGCTCAGCACTCAGAAGGCTGAGACAGGAG(+ACTCAC)GATCCTCCACAGAAACAGCGCTAGGCCAGCA NGG 10 8 17 8 24 0 100 5 10